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	Provedor de dados J. Venom. Anim. Toxins incl. Trop. Dis. (1) Autor Brandão-Filho,SP (1) Brito,MEFC (1) Gonçalves,SC (1) Paiva-Cavalcanti,M (1) Régis-da-Silva,CG (1) Palavra-chave Diagnosis (1) Glyceraldehyde-3-phosphate dehydrogenase (1) Leishmaniasis (1) Quality control (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2012 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis J. Venom. Anim. Toxins incl. Trop. Dis. Gonçalves,SC; Régis-da-Silva,CG; Brito,MEFC; Brandão-Filho,SP; Paiva-Cavalcanti,M. Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to develop multiplex PCR systems able to detect small amounts of target DNA of Leishmania infantum and Leishmania braziliensis, and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (G3PD) in mammals, enabling quality evaluation of the sample simultaneously with detection of the specific target. The systems created for G3PD recognition were combined with detection systems for L. infantum and L.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Diagnosis; Glyceraldehyde-3-phosphate dehydrogenase; Quality control. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200009 Registros recuperados: 1 Primeira ... 1 ... Última

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