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| Registros recuperados: 22 | |
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| Brandelli,Adriano; Riffel,Alessandro. |
| The strain Chryseobacterium sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37ºC, with maximum activity and yield at 30ºC. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30ºC and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Enzyme; Keratin; Protease. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100007 |
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| Seibert,Eduardo; Casali,Michel Elias; Leão,Marcos Laux de; Pezzi,Ernani; Brandelli,Adriano; Bender,Renar João. |
| No presente trabalho, foram avaliados os efeitos do hidrorresfriamento para manutenção da qualidade em pêssegos 'Chimarrita' e 'Chiripá'. O hidrorresfriamento foi realizado por imersão em água com gelo a 5ºC, sendo o abaixamento da temperatura monitorado por 3 minutos. O tratamento-controle foi transferência para a câmara fria imediatamente após a colheita dos pêssegos. Os frutos foram armazenados a 0ºC ('Chiripá') e 0,5ºC ('Chimarrita') por 7; 14 e 21 dias e avaliados na colheita, nas saídas de frio, seguidos de 2 a 3 dias a 20ºC para o amadurecimento. O hidrorresfriamento foi efetivo em retirar o calor de campo dos frutos. Os frutos hidrorresfriados desidrataram mais na armazenagem, e pêssegos de ambos os tratamentos apresentaram murchamento no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Frutas de caroço; Pré-resfriamento; Frigoconservação; Danos de frio. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452007000200028 |
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| Carli,Marcelo De; Bresolin,Bruna; Noreña,Caciano Pelayo Zapata; Lorini,Irineu; Brandelli,Adriano. |
| The effect of modified atmosphere packaging on the mortality of Sitophilus spp. in organic maize grain was studied. Maize grains were packed with five different atmospheres consisting of 20% O2, or 20, 40, 60, 80% CO2 with remaining N2. The packages were stored for 30 days at 26°C. CO2 and O2 concentrations were monitored inside the packages during the storage. The moisture content, titratable acidity and pH were analysed on the first and thirtieth day. At 5, 15 and 30 days of storage no significant differences were observed in the percentages of dead insects when CO2 was used. The results of progeny indicated that from the fifth day the number of emerging insects was low at 20, 60 and 80% CO2. Complete inhibition of the insects was achieved with 30 days... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carbon dioxide; Modified atmosphere; Insect mortality. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600024 |
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| Silva,Débora Scopel e; Castro,Clarissa Caetano de; Silva,Fábio da Silva e; Sant'anna,Voltaire; Vargas,Gilberto D'Avila; Lima,Marcelo de; Fischer,Geferson; Brandelli,Adriano; Motta,Amanda de Souza da; Hübner,Silvia de Oliveira. |
| P34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial peptides; Antiviral activity; Herpesvirus; Equine viral arteritis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300043 |
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| Bizani,Delmar; Brandelli,Adriano. |
| The presence of Aeromonas spp. in food has been demonstrated. They are often introduced from water, which is the natural habitat of many species and is thought to be the main source of contamination. The occurrence of Aeromonas spp. was investigated in 70 water samples of a bovine abattoir. Aeromonas spp. were present in 21.4% of water samples. A. hydrophila was isolated from 11.4% of supplying water samples and from 25.7% of the water drained from carcasses, whereas A. sobria was sole isolated from 5.7% of supplying water samples. Although greater number of positive samples were from water drained from carcasses, only A. hydrophila isolates were present. This suggests that the supplying water is the source of contamination. The antibiotic susceptibility... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas; Antibiotic activity; Aquatic environment; Hemolysis; Water. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400016 |
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| Oliveira,Sílvia Dias de; Bessa,Marjo Cadó; Santos,Luciana Ruschel dos; Cardoso,Marisa Ribeiro de Itapema; Brandelli,Adriano; Canal,Cláudio Wageck. |
| In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultry-related samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Rep-PCR; Phage typing; Antimicrobial resistance; Virulence genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400025 |
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| Corrêa,Ana Paula F.; Daroit,Daniel J.; Velho,Renata V.; Brandelli,Adriano. |
| The production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a Pseudomonas sp. belonging to the Pseudomonas jenssenii group, was studied. This bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). High levels of α-glucosidase were produced in skim milk broth. Hydrolytic enzymes detected in skim milk broth are of particular concern, indicating that these enzymes could be produced by Pseudomonas sp. during the cold storage of raw milk, contributing to the spoilage problem in milk and dairy products. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas; Hydrolases; Protease; Lipase; Glycosidase. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400034 |
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| Oliveira,Sílvia Dias de; Rodenbusch,Carla Rosane; Michael,Geovana B.; Cardoso,Marisa I.R.; Canal,Cláudio Wageck; Brandelli,Adriano. |
| The presence of three virulence genes, invA, spvR, and spvC, was determined in Salmonella Enteritidis isolated from poultry, pigs, humans and food. All isolates were positive for the invA gene, with 91.2% being positive for spvR and 90.2% for spvC. There was no significant difference in the prevalence of the virulence genes between isolates from different sources. The results indicate that there is a putative high virulence potential for the S. Enteritidis isolates characterized. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Virulence; Spv; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500042 |
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| Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano. |
| The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022 |
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| Riffel,Alessandro; Brandelli,Adriano. |
| The aim of this study was to characterize keratinolytic bacteria isolated from feather waste. Four isolates were selected after growth on solid medium with feather meal as sole carbon and nitrogen source and screened for proteolytic activity on milk agar plates. Three isolates were Gram-negative (belonged to the genera Burkholderia, Chryseobacterium and Pseudomonas) and one was Gram-positive (Microbacterium sp.). These bacteria grew on diverse keratin wastes such as feather meal, raw feathers, chicken nails, hair and wool. Keratinase activity was detected during growth, but the complete degradation of these substrates was not always achieved. The proteolytic character of crude enzymes was assessed using azokeratin and azocasein as substrates. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Feather; Keratin; Peptidase; Proteolysis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300036 |
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| Gontero,Mauro; Brandelli,Adriano; Zapata Noreña,Caciano. |
| The aim of this work was to produce crystallized fruit from watermelon rind. The following procedure was developed: the outer peel was removed; the material was sliced into 7 mm cubes, blanched for, 0, 5 and 10 minutes, and then treated with 10% sodium chloride solution. This product was treated with solutions of sucrose (30 to 72 °Brix), and dried in a hot air dryer at different temperatures (40, 60 and 80 °C). Products were then analyzed by a sensory panel. The experimental design used was randomized blocks and the results were analyzed by the Tukey's test. The best acceptance of the sensory panel was for the product obtained by 5 minutes blanching followed by drying at 60 °C, whose intensity values for appearance, flavor and gummosis were between 6 and 7. |
| Tipo: Journal article |
Palavras-chave: Osmosis; Watermelon; Dehydration; Agricultural waste. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000200006 |
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| Castro,Clarissa Caetano de; Silva,Débora Scopel e; Costa,Géssica Aracéli; Fischer,Geferson; Vargas,Gilberto D’Avila; Brandelli,Adriano; Lima,Marcelo de; Motta,Amanda de Souza da; Hübner,Silvia de Oliveira. |
| ABSTRACT: Previous studies have demonstrated the antimicrobial activity of the peptide P34. In this study, the antiviral potential of P34 and the in vitro mechanism of action were investigated against bovine alphaherpesvirus type 1 (BoHV1). P34 exhibited low toxicity, a high selectivity index (22.9) and a percentage of inhibition of up to 100% in MDBK cells. Results from antiviral assays indicated that P34 did not interact with cell receptors, but it was able to inhibit the viral penetration immediately after pre-adsorption. In addition, BoHV1 growth curve in MDBK cells in the presence of P34 revealed a significant reduction in virus titer only 8h post-infection, also suggesting an important role at late stages of the replicative cycle. Virucidal effect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antiviral; Herpesvirus; Peptide. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600451 |
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| Santos,Helena Caminha; Brandelli,Adriano; Ayub,Marco Antônio Záchia. |
| In this work, analysis of chicken breast fillets was performed for shear values and sensory evaluation after different times of aging. Aging process was carried out by holding carcasses in covered plastic boxes, in a cold room, at 3 +/- 1ºC; groups of 5 carcasses were stored and classified according to the period of storage in cold room as 0 (control), 2, 4, 8, 12 and 24 hours. The variation in pH of chicken carcasses during rigor decreased from 6.4 to 5.9. However, during the process of aging, the pH values increased up to 6.2 in the first 12h, probably as the result of proteolysis. The Warner-Bratzler shear values decreased as aging time increases, resulting in a first order kinetic. The carcasses presented a more accentuated decline in shear values in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken breasts; Meat tenderization; Sensory evaluation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300038 |
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| Canto,João Ignácio do; Neves,Jairo Pereira; Gonçalves,Paulo Bayard Dias; Oliveira,João Francisco; Moraes,José Carlos Ferrugem; Ceccim,Marcelo; Brandelli,Adriano. |
| Com o objetivo de avaliar o efeito do desmame interrompido na dinâmica folicular aos 35 e 70 dias pós-parto, 15 vacas da raça Charolesa com parição na primavera, manejadas em campo nativo, foram distribuídas deforma aleatória em cinco diferentes tratamentos de desmame como segue: uso de tabuleta por dois dias (TAB 2), quatro dias (TAB 4), separação total por dois dias (TOT 2), quatro dias (TOT 4) e controle. A partir do início dos tratamentos, foi realizado monitoramento da atividade ovariana durante oito dias, e coletas diárias de sangue para dosagem de progesterona e identificação de cio com auxílio de rufião. Os ovários foram avaliados ecograficamente e os folículos foram classificados, conforme o diâmetro, em pequenos (< 3mm), médios (4 a 9mm) e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anestro pós-parto; Desmame interrompido; Dinâmica folicular. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781998000400019 |
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| Registros recuperados: 22 | |
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