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| Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie. |
| Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved. |
| Tipo: Text |
Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf |
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| Briand, Elisabeth; Salmain, Michèle; Compere, Chantal; Pradier, Claire-marie. |
| Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal... |
| Tipo: Text |
Palavras-chave: PM IRRAS; Antibody; Protein A; Gold; Self assembled monolayers. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2222.pdf |
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| Briand, Elisabeth; Salmain, M; Compere, Chantal; Pradier, C. |
| Two antibody immobilisation procedures were compared to set up an immunosensor for goat anti-rabbit immunoglobulin (anti-rIgG), i.e. rIgG covalently bound or immobilised via affinity to protein A (PrA). In both cases, the first layer of protein was covalently bound to a mixed self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) on a gold surface. The elaboration of the sensitive surfaces, as well as their selectivity and sensitivity were studied step by step by polarization modulation-reflection absorption infra-red spectroscopy (PM-RAIRS) and quartz crystal trticrobalance (QCM) with impedance measurement. QCM measurements showed that the viscoelastic properties of the antibody layer were markedly modified during the... |
| Tipo: Text |
Palavras-chave: RIgG immobilisation; PM RAIRS; QCM; Immunosensors. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3580.pdf |
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