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Phenotypical characteristics of group B streptococcus in parturients BJID
Simoes,Jose Antonio; Alves,Valeria Moraes Neder; Fracalanzza,Sergio Eduardo Longo; Camargo,Rodrigo Pauperio Soares de; Mathias,Lenir; Milanez,Helaine Maria Besteti Pires; Brolazo,Eliane Melo.
Colonization by Group B Streptococcus (GBS) is highly prevalent among pregnant women, with prevalence rates ranging between 4% and 30%. The infection may be transmitted vertically and may result in serious neonatal consequences. In the period from November 2003 to May 2004, a cross-sectional study was carried out among 316 parturients at the Jundiaí Teaching Hospital to establish the prevalence of genital GBS colonization, to identify the factors associated with colonization and the characteristic phenotypes of these streptococci. Samples from rectal and vaginal areas were collected for selective culture in Todd-Hewitt broth. Susceptibility to 7 antimicrobial agents was tested using the antibiotic diffusion disk technique, and the isolated strains were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus agalactiae; Neonatal infections; Antimicrobial resistance; Serum type; GBS; Pregnant woman.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200019
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Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates BJM
Brolazo,Eliane Melo; Leite,Domingos Silva; Tiba,Monique Ribeiro; Villarroel,Marina; Marconi,Camila; Simoes,Jose Antonio.
Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii (25%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrate fermentation test; Multiplex PCR; Identification; Vaginal lactobacilli; Vaginal ecosystem.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028
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