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Molecular diversity of human parvovirus B19 during two outbreaks of erythema infectiosum in Brazil BJID
Garcia,Rita de Cássia Nasser Cubel; Pereira,Renata Freire Alves; Azevedo,Kátia Martins Lopes de; Castro,Tatiana Xavier de; Mello,Francisco C.A.; Setubal,Sérgio; Siqueira,Marilda M.; Brown,David; Oliveira,Solange Artimos de.
Abstract This study was conducted to provide information on the genetic diversity of human parvovirus B19 (B19V) circulating in the municipality of Niterói, Rio de Janeiro, Southeast Brazil during 1996–2006, a period with two distinct outbreaks of B19V infection: 1999–2000 and 2004–2005. A total of 27 sera from patients with erythema infectiosum and five sera from HIV-infected patients that tested positive for B19V DNA during the study period were analyzed. To genotype B19V strains, a semi-nested PCR for partial amplification of the capsid gene was performed and sequence analysis revealed that 31 sequences belonged to subgenotype 1a (G1a) of the main genotype 1 and one sequence was characterized as subgenotype 3b (G3b). The phylogenetic tree supported the...
Tipo: Info:eu-repo/semantics/other Palavras-chave: B19V genotypes; Outbreak; Erythema infectiosum; Brazil.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100102
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