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Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis BJM
Santos,Elisane Odriosolla dos; Michelon,Mariano; Furlong,Eliana Badiale; Burkert,Janaína Fernandes de Medeiros; Kalil,Susana Juliano; Burkert,Carlos André Veiga.
The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Raw glycerol; Single cell protein; Yeast biomass; Yarrowia lipolytica.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200002
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RICE PARBOILING WASTEWATER IN THE MAXIMIZATION OF CAROTENOIDS BIOPRODUCTION BY Phaffia rhodozyma Ciência e Agrotecnologia
Rios,Danielle Alves da Silva; Borba,Thais de Matos de; Kalil,Susana Juliano; Burkert,Janaína Fernandes de Medeiros.
The interest in carotenoid production from natural sources has increased based in their possible use as dyes and their powerful biological antioxidant capacity. This study evaluated the use of rice parboiling wastewater (RPW) as an alternative substrate for the bioproduction of carotenoids using the yeast Phaffia rhodozyma and found it to be promising as the only source of nutrients, reaching a concentration of 0.6 μg mL-1 (259.1 μg g-1) in 48 h. To increase the potential use of this industrial effluent, a study of supplementation was carried out to enhance the production of carotenoids using the methodology of experimental design (a 2IV 6-2 fractional factorial design sequenced by a 24 central composite design). The conditions for maximizing the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodye; Submerged cultive; Response surface methodology; Yeast.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000400401
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Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content Ciência e Agrotecnologia
Martins,André Rosa; Monteiro,Ricardo Lemos; Burkert,Janaína Fernandes de Medeiros; Burkert,Carlos André Veiga.
In a single-stage process with the simultaneous addition of β-galactosidase and lactic culture, the lactose conversion, the processing time, viscosity and syneresis were evaluated. Fermentation was promoted by lactic culture containing two probiotic microorganisms, Bifidobacterium animalis and Lactobacillus acidophilus, associated with the typical microorganisms of yogurt. An enzymatic preparation containing β-galactosidases from Kluyveromyces lactis and Aspergillus niger was used. A central composite design (2³ trials plus three central points) was proposed in order to evaluate the effects of initial lactose concentration, enzyme concentration and the time of addition of the enzyme. The following conditions were established: initial lactose concentration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic catalysis; Lactose hydrolysis; Yogurt physical properties; Β-galactosidase.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000500008
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