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Morphological variability of Bathybdella sawyeri (Hirudinida : Piscicolidae) from hydrothermal vents on the Galapagos Rift and the South East Pacific Rise ArchiMer
Burreson, Eugene; Segonzac, Michel.
The deep-sea fish leech Bathybdella sawyeri Burreson has recently been collected from two sites on the South East Pacific Rise at approximately 14 degrees and 17 degrees S, 2623 - 2586 m depth. Leeches were obtained from washings of vent mussels Bathymodiolus thermophilus (Mytilidae) and clams Calyptogena magnifica (Vesicomyidae) collected with a grab by a submersible, or were found attached to the cheliped of the crab Cyanagraea praedator (Bythograeidae) collected in baited traps at 14 S. Most individual leeches had morphology typical of B. sawyeri as originally described, with suckers of approximately equal size, but all specimens collected from the crab C. praedator had unusually large oral suckers that were distinctly larger than the caudal sucker....
Tipo: Text Palavras-chave: Cyanagraea; Bythograeidae; Bathybdella; Hydrothermal vent; East Pacific Rise; Piscicolidae; Hirudinida.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2049.pdf
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Use of molecular tools for mollusc disease diagnosis ArchiMer
Berthe, Franck; Burreson, Eugene; Hine, Mike.
Recently, an increasing number of research teams are engaged in developing DNA-based diagnostic techniques for mollusc pathogens. These techniques are slowly moving from development in specialised laboratories for research purposes, to routine application and are expected to find an increasing use in routine disease monitoring programs in mollusc aquaculture and in efforts to prevent the spread of pathogens within and between nations. Considering the probable development and interest in these diagnostic techniques, the potential for wide applications in aquaculture and the inherent problems currently associated with their use, it appears necessary to address these issues.
Tipo: Text Palavras-chave: Molecular tools; Mollusc disease; Diagnosis; Pathology.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-3291.pdf
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Haplosporidiosis in the pacific oyster Crassostrea gigas from the French Atlantic coast ArchiMer
Renault, Tristan; Stokes, Nancy; Chollet, Bruno; Cochennec, Nathalie; Berthe, Franck; Gerard, Andre; Burreson, Eugene.
Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A...
Tipo: Text Palavras-chave: Haplosporidium nelsoni; Haplosporidiosis; Crassostrea gigas; Pacific oyster.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-821.pdf
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