|
|
| Casablancas,Antoni; Cárdenas-Fernández,Max; Álvaro,Gregorio; Benaiges,Maria Dolors; Caminal,Glòria; Mas,Carles de; González,Glòria; López,Carmen; López-Santín,Josep. |
| Background: New enzymes for biotransformations can be obtained by different approaches including directed mutagenesis and in vitro evolution. These mutants have to be efficiently produced for laboratory research on bioreactions as well as for process development. In the framework of a European ERA-IB project, two different types of enzymes (ammonia lyases and aminotransferases) have been selected as biocatalysts for the synthesis of industrially relevant amines. New mutant enzymes have been obtained: a) aspartases able to recognize β-amino acids; b) ω-transaminases with improved activity. The objectives are to find out a common operational strategy applicable to different mutants expressed in E. coli with the same initial genetic... |
| Tipo: Journal article |
Palavras-chave: Amine transaminases; Ammonia lyases; E. coli; Fed-batch; Immobilized biocatalysts. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300004 |
| |
