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| Silveira,Marcelo Marques da; Cândido,Stéfhano Luis; Santos,Karin Rinaldi dos; Maia,Maerle Oliveira; Souza,Roberto Lopes de; Sousa,Valéria Régia Franco; Almeida,Arleana do Bom Parto Ferreira de; Dutra,Valeria; Nakazato,Luciano. |
| ABSTRACT: Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood culture; PCR; Bacteremia; Fungemia. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600452 |
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