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Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans Biol. Res.
CHIUCHETTA,SIMONE JUREMA RUGGERI; CASTRO-PRADO,MARIALBA AVEZUM ALVES DE.
Vincristine is an alkaloid widely used as an antineoplastic agent. In eukaryotic cells the drug causes blockage in the G2 phase of the cell cycle and an increase in the frequency of sister chromatid exchanges. Due to the fact that germinating Aspergillus nidulans cells spend most of their cycle in G2 phase, they provide an excellent system for the study of mitotic crossing-over. Taking into account that mitotic crossing-over occurs during G2 period, the evaluation of recombinagenic and aneugenic potential of vincristine is provided with regard to two diploid strains of A. nidulans: a wild strain (uvsH+//uvsH+) and a defective one in DNA repair (uvsH//uvsH). Drug toxicity and its effect on the asexual cycle of A. nidulans has been evaluated as well....
Tipo: Journal article Palavras-chave: Aneuploidy; Antineoplastic drug; Aspergillus nidulans; Recombinagenic activity.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100006
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