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MOLECULAR VARIABILITY OF THREE GENES OF POTATO VEIN YELLOW VIRUS INFECTING Solanum tuberosum, USING SINGLE STRAND CONFORMATIONAL POLYMORPHISM Acta biol.Colomb.
CUBILLOS-ABELLO,Karen Andrea; GUZMÁN BARNEY,María Mónica.
Potato yellow vein virus (PYVV), a virus with tripartite RNA (ss+) genome is classified as member of the genus Crinivirus within the family Closteoviridae. PYVV is the causal agent of potato yellow vein disease (PYVD) with yield loss between 25 %-50 % in field. Single strand conformational polymorphism (SSCP) has been reported to estimate variability in different viruses' species. In this study, the molecular variability of PYVV analyzed by SSCP patterns of three genes: major capsid protein (CP), minor capsid protein (CPm), and heat shock protein (Hsp70) obtained from 60 virus isolates from potato plants expressing PYVD. Leaves collected in Nariño, Colombia from 30 Solanum tuberosum Phureja Group (PhG) and 30 from the Andigena Group (AG). Genes amplified...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Closteroviridae; Potato; Variability; Viral proteins.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100026
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