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Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk Biol. Res.
SOUZA VIANA,ELISETH; MARTINO CAMPOS,MARIA EMILENE; REIS PONCE,ADRIANA; CUQUETTO MANTOVANI,HILÁRIO; DANTAS VANETTI,MARIA CRISTINA.
The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy....
Tipo: Journal article Palavras-chave: Acyl homoserines lactones; Biofilm; Furanones; Quorum sensing.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400004
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