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| Diniz-Sousa,Rafaela; Kayano,Anderson M.; Caldeira,Cleópatra A.; Simões-Silva,Rodrigo; Monteiro,Marta C.; Moreira-Dill,Leandro S.; Grabner,Fernando P.; Calderon,Leonardo A.; Zuliani,Juliana P.; Stábeli,Rodrigo G.; Soares,Andreimar M.. |
| Abstract Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis. Methods: P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wasp; Polybia occidentalis; PocTX; PLA2 homologue. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100302 |
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| Oliveira,Cláudia S.; Caldeira,Cleópatra A. S.; Diniz-Sousa,Rafaela; Romero,Dolores L.; Marcussi,Silvana; Moura,Laura A.; Fuly,André L.; Carvalho,Cicília de; Cavalcante,Walter L. G.; Gallacci,Márcia; Pai,Maeli Dal; Zuliani,Juliana P.; Calderon,Leonardo A.; Soares,Andreimar M.. |
| Abstract Background: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caribbean sea cnidarians; Bioprospection; Antiophidic; Antitumor; Natural products. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100313 |
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