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Molecular characterization of virulence genes cctA, nanA, and fliC in Clostridium chauvoei from Rio Grande do Sul and São Paulo State, Brazil 65
Ziech,Rosangela Estel; Farias,Luana D’Avila; Ardisson-Araújo,Daniel Mendes Pereira; Camargo,Gregório Miguel Ferreira de; Frey,Joachim; Vargas,Agueda Castagna de.
ABSTRACT: Clostridium chauvoei toxin A (CctA), neuraminidase (NanA), and flagellin (FliC) proteins contribute to the pathogenicity of Clostridium chauvoei, the causative agent of blackleg in cattle. The aim of this study was to analyze the genetic variability of cctA, nanA, and fliC genes in C. chauvoei isolates from the Rio Grande do Sul and São Paulo state- Brazil, during different sampling periods. The presence of these genes was verified through PCR amplification and partial gene sequencing of 17 strains. Alignment of PCR amplicons combined with bioinformatics analysis was used in an attempt to study the variability across C. chauvoei solates. The similarity among the partial sequences of cctA and nanA genes was 100%. The sequencing of fliC revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blackleg; Clostridium chauvoei toxin A; Neuraminidase; Flagellin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500452
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Genetic parameters for milk, fat and protein yields in Murrah buffaloes (Bubalus bubalis Artiodactyla, Bovidae) 74
Aspilcueta-Borquis,Rusbel Raúl; Sesana,Roberta Cristina; Berrocal,Milthon Honorio Munoz; Seno,Leonardo de Oliveira; Bignardi,Annaiza Braga; El Faro,Lenira; Albuquerque,Lucia Galvão de; Camargo,Gregório Miguel Ferreira de; Tonhati,Humberto.
The objective of the present study was to estimate genetic parameters for test-day milk, fat and protein yields and 305-day-yields in Murrah buffaloes. 4,757 complete lactations of Murrah buffaloes were analyzed. Co-variance components were estimated by the restricted maximum likelihood method. The models included additive direct genetic and permanent environmental effects as random effects, and the fixed effects of contemporary group, milking number and age of the cow at calving as linear and quadratic covariables. Contemporary groups were defined by herd-year-month of test for test-day yields and by herd-year-season of calving for 305-day yields. The heritability estimates obtained by two-trait analysis ranged from 0.15 to 0.24 for milk, 0.16 to 0.23 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Test-day model; Accumulated productions; Heritability; Genetic correlations.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100014
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