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Evaluation of hela cell lineage response to β radiation from Holmium-166 embedded in ceramic seeds BABT
Valente,Eduardo Sarmento; Cuperschmid,Ethel Mizrahy; Campos,Tarcisio Passos Ribeiro de.
This work studied the effects of β radiation of Ho-166 embedded in ceramic seeds on HeLa cells. Methodology consisted in the production of ceramic seeds with holmium-165 by sol-gel route. Chemical and physical characterizations of the seeds were performed. Subsequently, nuclear characterization was performed by gamma spectrometry. Experimental and theoretical activities were defined and initial dose rate were evaluated by MIRD (Medical Internal Radiation Dose Committee) methodology. The seeds were placed in confluent culture flasks and remained for six radionuclide half-lives. Biological results were represented by a clean 6 mm diameter area around the seed where the tumour cells were killed. The initial dose rate was 15.5 Gy. h-1. The maximum absorbed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachytherapy; Ceramic seeds; Holmium; Cancer control; HeLa cells.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500013
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In vitro response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to 60Co at single fraction BABT
Andrade,Lídia Maria; Campos,Tarcisio Passos Ribeiro de; Leite,M. F.; Góes,A. M..
Radiotherapy using gamma rays is a common modality of breast cancer treatment. The aim of this research is to investigate the biological response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells (PBMC) exposed in vitro to 60 Co irradiation at a single fraction of 10 Gy, 25 Gy and 50 Gy doses at 136,4 cGy.min-1 rate. Cells were irradiated at room temperature by the Theratron 80 radiotherapy system. Biological response was evaluated through cellular viability using MTT assay and nucleus damages visualized by Propidium Iodide assay and electrophoresis agarose gel after gamma irradiation. Nucleus damages induced by 60Co irradiation were compared to damage caused by cell exposure to 10% methanol. The 50 Gy dose of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breast Cancer; MDAMB-231; PBMC and Gamma rays.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700031
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The surgical viability and radiological monitoring of brain implants of bioactive micro-seeds in an animal model BABT
Silva,Giane X. O.; Campos,Tarcisio Passos Ribeiro de; Siqueira,Sávio Lana; Maciel,Marcelo B..
The interstitial implant is a therapeutic modality in brachytherapy of the head and neck. Presently, the seeds implanted in tumors in the central nervous system are metallic I-125. After the full emission of the radionuclide, the seed remains inert in the implanted area. Bioactive ceramic seeds have been prepared for this research group incorporating Sm-152 to be active in Sm-153. The main goal of the present study is the development of a the surgical technique for implanting the biodegradable radioactive micro-seeds in the brains of rabbits, as well as the observation of the clinical reactions of the animal after implantation of two sets of three seeds. The surgical procedure consisted of performing two separate perforations 10 mm from each other in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BrainImplant; Surgery; Bioactive seeds; Sm-153.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700016
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Gd-GLU toward NMR imaging: synthesis, characterization and breast cell uptake assay BJPS
Campos,Tarcisio Passos Ribeiro de; Dalmazio,Ilza; Augusti,Rodiney; Almeida,Iassudara Garcia.
Breast cancer cell uptake of Gd-metal is investigated based on the formation of coordinate compounds of gadolinium and glucose (Glu) molecules in solution. The hypothesis is that glucose helps Gd-internalization by complex formations constituted of Gd3+ coordinate to m-glucose molecules, whose valence was complemented by Cl- anions. Such a proposal is an insight toward a metabolic-dependent contrast-agent for cancer and inflammation in magnetic resonance image. A solution was prepared based on anhydrous d-glucose and gadolinium chloride (Gd-Glu). Uptake assays for MDA-MB-231(c231) cells were elaborated collecting incubated c231-cells with Gd-Glu and measuring metal-uptake and their concentrations by Nuclear Activation Analysis (NAA). The ionic solution was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose; Gadolinium; Breast cancer; In vitro uptake; MDA-MB-231.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100527
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