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Copper-induced adaptation, oxidative stress and its tolerance in Aspergillus niger UCP1261 Electron. J. Biotechnol.
Cavalcanti Luna,Marcos A; Rodrigues Vieira,Edson; Okada,Kaoru; Campos-Takaki,Galba Maria; do Nascimento,Aline Elesbão.
Background The effects of exposure to copper, during growth, on the production of biomass, total protein, catalase, glutathione-S transferase, glutathione peroxidase, peroxidase, polyphosphate, acid and alkaline phosphatases, ultrastructure and the ability to remove this metal from Aspergillus niger, obtained from caatinga soil, were evaluated. Results All parameters tested were influenced by the concentration of metal in the culture medium. The presence of metal induced high levels of antioxidant enzymes, including lipid peroxidation, thereby revealing the appearance of an oxidative stress response. The variation in polyphosphate levels indicates the participation of the polymer in response to stress induced by copper. The activities of the phosphatases...
Tipo: Journal article Palavras-chave: Aspergillus niger; Antioxidant enzyme; Copper removal; Polyphosphate; Ultrastructure.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600006
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Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor BABT
Porto,Ana Lúcia Figueiredo; Sarubbo,Leonie Asfora; Moreira,Keila Aparecida; Melo,Homero José Farias de; Lima-Filho,José Luís; Campos-Takaki,Galba Maria; Tambourgi,Elias Basile.
A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Efficiency; Mass transfer coefficient; Perforated Rotating Disc contactor; Aqueous two-phase system; Ascorbic oxidoreductase.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019
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Monitoring the effect of pyrene on the germination and radial growth of the wild and mutant strains of Rhizopus arrhizus UCP402 BABT
Shiosaki,Ricardo Kenji; Albuquerque,Clarissa Daisy da Costa; Okada,Kaoru; Fukushima,Kazutaka; Campos-Takaki,Galba Maria.
The physiological mutant of Rhizopus arrhizus was obtained in the pyrene resistance gradient test. Comparative studies were carried out about the behavior of the germination process and the radial growth of the mutant and wild strains of R. arrhizus UCP 402. Sabouraud Sucrose and Yeast Malt Broth cultures containing pyrene (10 mg/L) induced the germination process of the sporangiospores of the wild and mutant strains of R. arrhizus. The radial growth of the strains was inversely proportional to the pyrene concentration in the culture medium. The results showed an adaptation of R. arrhizus UCP 402x (mutant) in the pyrene (50mg/L) and suggested a higher ability of application in the removal of pyrene from the contaminated areas.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pyrene; Rhizopus arrhizus; Mutant; Radial growth; Germination.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300024
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A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium BJM
Nascimento,Aline E.; Shariá,Allana E. do Nascimento; Lima,Marco Antonio Barbosa de; Campos-Takaki,Galba Maria.
Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipolytica; Carbohydrate cytochemistry; Fungi ultrastructure.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008
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