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Development and validation of new SSR markers from expressed regions in the garlic genome Scientia Agricola
Ipek,Meryem; Sahin,Nihan; Ipek,Ahmet; Cansev,Asuman; Simon,Philipp W..
Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs) at the publicly available GarlicEST database were screened for SSR motifs and a total of 132 SSR motifs were identified. Primer pairs were designed for 50 SSR motifs and 24 of these primer pairs were selected as SSR markers based on their consistent amplification patterns and polymorphisms. In addition, two SSR markers were developed from the sequences of garlic cDNA-AFLP fragments. The use of 26 EST-SSR markers for the assessment of genetic relationship was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags (EST); Simple sequence repeat markers; Genetic relationship.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100041
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