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Genetic diversity in populations of Maytenus dasyclada (Celastraceae) in forest reserves and unprotected Araucaria forest remnants Acta Botanica
Reichmann,Manuel Castilhos; Zanella,Camila Ângela; Valério Júnior,Cláudio; Borges,Ana Claudia Piovezan; Sausen,Tanise Luisa; Paroul,Natalia; Mielniczki-Pereira,Albanin Aparecida; Teixeira,Amito José; Budke,Jean Carlos; Mossi,Altemir José; Cansian,Rogério Luis.
ABSTRACT Understanding the genetic structure and diversity of plants is fundamental to their conservation and permits their sustainable use by local communities. The genus Maytenus (Celastraceae) is composed of plants possessing pharmacological and antioxidant properties. However, the genetic and economic properties of the species M. dasyclada, a typical species of Araucaria forests in Brazil and Uruguay, have been little studied. In this work, the genetic structure and diversity of natural populations of M. dasyclada located in unprotected and preserved forest remnants were investigated using RAPD and isozymes markers. The results demonstrated that in areas of preservation, populations of M. dasyclada possess a relatively high degree of polymorphism and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Forest reserves; Genetic diversity; Maytenus dasyclada; Molecular markers.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100093
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Decontamination of Pig Carcasses Using Water Pressure and Lactic Acid BABT
Brustolin,Jean Carlos; Dal Pisol,Andreia; Steffens,Juliana; Toniazzo,Geciane; Valduga,Eunice; Di Luccio,Marco; Cansian,Rogério Luis.
The objective of this work was to evaluate the effect of different water pressures and concentrations of lactic acid on microbial counts (mesophilic bacteria, enterobacteria and Salmonella) on pig carcasses without contamination and contaminated carcasses, before and after the last shower and before being cooled. The tests were carried out using 4, 3 and 2 bar water pressure, and 2, 1 and 0% lactic acid concentration. In general, both the pressure in the shower and lactic acid had a positive effect by reducing the microbial count. The interaction between the pressure and lactic acid caused the largest reduction in carcasses surface count for mesophiles. With regard to enterobacteria on contaminated carcasses, the most important variable was the lactic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pig carcasses; Decontamination; Water pressure; Lactic acid; Bacteria.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600954
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Microbiological analysis of critical points in the chicken industry BABT
Cansian,Rogério Luis; Floriani,Salete Teresa Radeski; Valduga,Eunice.
This work is focused on identifying microbial contamination in the scalding asepsis and cooling processes as well as in fresh sausages obtained. Salmonella was identified in two scald water samples but was absent in the water from chiller and in the final product, which might be explained in terms of chlorine addition and temperature reduction. The analysis revealed that MPN of Escherichia coli was in the range of <1-11/mL in the scald water and <1-64/mL in the chiller. Aeromonas sp. count showed 5 to 3.5x10¹ CFU/mL in the scald water and 9 to 3.7x10² CFU/mL in the chiller, probably due to the psycrophile characteristics of Aeromonas and evisceration process. The analysis of fresh sausages chicken revealed a sharp increase in the Aeromonas count...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Bacteria; Chicken; Microbiology; Food quality control.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300011
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Semi-volatile compounds variation among Brazilian populations of Ilex paraguariensis St. Hil. BABT
Cansian,Rogério Luis; Mossi,Altemir José; Mazutti,Márcio; Oliveira,José Vladimir; Paroul,Natalia; Dariva,Cláudio; Echeverrigaray,Sérgio.
The use of compressed carbon towards extracting semi-volatile compounds present in maté leaves (Ilex paraguariensis St. Hil.) is due to the growing interest in mate constituents to develop new products in the cosmetic, pharmaceutical and food industries. The objective of this work was to assess the chemical distribution of semi-volatile compounds in 20 native populations of maté collected all over Brazil. The extracts of bulk samples (30 plants) of each population were obtained by the high-pressure carbon dioxide extraction technique, and analyzed by GC/MSD. The quantification of compounds (caffeine, theobromine, phytol, squalene, vitamin E, eicosane, pentatriacontane, and stigmasterol) showed significant variations within the different populations and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maté; Chemical variability; Brazilian native populations; Supercritical extraction.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100021
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Evaluation of Origanum vulgare essential oil as antimicrobial agent in sausage BJM
Busatta,Cassiano; Mossi,Altemir José; Rodrigues,Maria Regina Alves; Cansian,Rogério Luis; Oliveira,José Vladimir de.
This work reports antimicrobial activity of oregano (Origanum vulgare) essential oil against several bacteria in sausage. The in vitro minimum inhibitory concentration (MIC) was determined for 9 selected aerobic heterotrofic bacteria. The antimicrobial activity of distinct concentrations of the essential oil on the basis of the highest MIC found was tested in a food system comprised of fresh sausage. Batch food samples were also inoculated with Escherichia coli with a fixed concentration and the time course of the product was evaluated with respect to the action of the different concentrations of essential oil. Sensory analysis were conducted, and results showed that the addition of oregano essential oil to sausage may be a promising route as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial properties; Oregano (Origanum vulgare); Food system; Fresh sausage.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400006
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The effects of acute administration of the hydroalcoholic extract of rosemary (Rosmarinus officinalis L.) (Lamiaceae) in animal models of memory BJPS
Zanella,Camila Angela; Treichel,Helen; Cansian,Rogério Luis; Roman,Silvane Souza.
Rosmarinus officinalis (Rosemary) demonstrates antioxidant, antidepressant, diuretic, antinociceptive and antiulcerogenic activities. The present study was designed to examine the effects of the hydroalcoholic extract of R. officinalis on the memory of male mice. The behavioral tasks employed were social recognition (SR), the Morris water maze (MWM) and an inhibitory avoidance task (IA). The treatment with 150 and 300 mg/kg of R. officinalis improved the acquisition phase of learning of a new social memory in the SR task because a decrease was observed in the duration of social investigation. In the Morris water maze, no significant effect was observed on spatial memory when the groups were compared for the time spent in the correct quadrant. In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rosmarinus officinalis/pharmacognosy; Rosmarinus officinalis/hydroalcoholic extract; Rosmarinus officinalis/experimental study; Short-term memory/experimental study; Long-term memory/experimental study; Spatial memory/experimental study.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300005
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Alterações na qualidade de raízes de mandioca(Manihot esculenta Crantz) minimamente processadas Ciência e Agrotecnologia
Alves,Andreia; Cansian,Rogério Luis; Stuart,Giane; Valduga,Eunice.
A conservação pós-colheita das raízes de mandioca tem sido uma preocupação das indústrias e produtores, devido ao curto tempo de estocagem e a alta perecibilidade das raízes. Dois fenômenos são apontados como responsáveis pela deterioração das raízes, um de ordem fisiológica, provocando a perda inicial da qualidade por meio do desenvolvimento da descoloração vascular do tecido parenquimatoso, e o outro, de ordem microbiana, que se segue à fisiologia, responsável pela decomposição do produto. Dessa forma, com o presente trabalho, objetivou-se estudar a conservação das raízes de mandioca (Manihot esculenta Crantz), submetidas à higienização em água clorada e armazenadas em três tipos de embalagens, bandeja de isopor envolta em filme de policloreto de vinila...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mandioca; Processamento mínimo; Embalagem.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000200009
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Influence of different sanitizers on food contaminant bacteria: effect of exposure temperature, contact time, and product concentration Ciênc. Tecnol. Aliment.
Beltrame,Cezar Augusto; Kubiak,Gabriela Busnello; Lerin,Lindomar Alberto; Rottava,Ieda; Mossi,Altemir José; Oliveira,Débora de; Cansian,Rogério Luis; Treichel,Helen; Toniazzo,Geciane.
The efficiency of four Sanitizers - peracetic acid, chlorhexidine, quaternary ammonium, and organic acids - was tested in this work using different bacteria recognized as a problem to meat industry, Salmonella sp., S. aureus, E. coli and L. monocytogenes. The effects of sanitizer concentration (0.2, 0.5, 0.6, 1.0, 1.1 and 1.4%), at different temperatures (10 and 45 °C) and contact time (2, 10, 15, 18 and 25 minutes) were evaluated. Tests in an industrial plant were also carried out considering previously obtained results. In a general way, peracetic acid presented higher efficiencies using low concentration (0.2%) and contact time (2 minutes) at 10 °C. The tests performed in industrial scale showed that peracetic acid presented a good performance in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sanitizers; Bacteria; Food industries.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200003
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Ocorrência de Bacillus sporothermodurans em leite UAT integral e desnatado Ciênc. Tecnol. Aliment.
Busatta,Cassiano; Valdruga,Eunice; Cansian,Rogério Luis.
Por sua composição completa e balanceada, o leite é um substrato ideal para o desenvolvimento de diversos grupos de microrganismos. Bactérias, fungos, vírus e outros podem provocar significativas alterações no leite e derivados. Apesar do tratamento a Ultra Alta Temperatura a que o leite longa vida é submetido, constatou-se nos últimos anos, a presença de um novo tipo de bactéria altamente resistente ao calor, denominada Bacillus sporothermodurans, em função de sua capacidade de formação de esporos. O presente trabalho teve como objetivos verificar a presença desta bactéria em leites UAT (Ultra Alta Temperatura) integral e desnatado, comercializados na região do Alto Uruguai - RS e quantificar a capacidade de resistência à altas temperaturas das formas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus sporothermodurans; UHT; UAT; Endoesporos.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000300003
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Microorganisms screening for limonene oxidation Ciênc. Tecnol. Aliment.
Lerin,Lindomar; Toniazzo,Geciane; Oliveira,Débora de; Rottava,Leda; Dariva,Cláudio; Cansian,Rogério Luis; Treichel,Helen; Padilha,Francine; Antunes,Octávio Augusto Ceva.
Limonene is a monoterpene obtained in large amounts from essential oils and is used as a raw material for the synthesis of flavors and fine chemicals. Several pathways or routes for the microbial degradation of limonene making use of the cytochrome P450-dependent monooxygenases have been described. In this study, we present a fermentative screening of microorganisms in order to verify their ability to perform the desirable conversion. In parallel, the PCR technique was used to select the microorganisms that contain the limC gene, which is responsible for the conversion of carveol to carvone. The microorganisms selected by PCR were not able to bioconvert limonene. From this result, we can suppose that these strains do not have the gene that codifies the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDH (carveol dependent dehydrogenase); Carveol; Carvone; PCR; Fermentative screening.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200017
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Lipid and protein oxidation in the internal part of italian type salami containing basil essential oil (Ocimum basilicum L.) Ciênc. Tecnol. Aliment.
Cichoski,Alexandre José; Cansian,Rogério Luis; Oliveira,Débora de; Gaio,Iloir; Saggirato,Adriana Galon.
Different concentrations of basil essential oil (Ocimum basilicum L.) (0.19; 0.38; 0.75; 1.87; 3.75 and 6.00 mg.g-1) were evaluated in relation to their antioxidant activity using the DPPH● radical methodology. From the IC50 obtained data, the concentrations of 0.19; 0.38; 0.75; 1.87; 3.75; 6.00 and 12.00 mg.mL-1 were applied directly to the product and these were sensorially evaluated by the test of control difference. The concentrations related to the highest acceptability (0.19; 0.38 and 0.75 mg.g-1) were tested for antioxidant activity in the internal part of Italian type salami - during the processing and after 30 days of storage, in terms of lipid and protein oxidation. The oxidation of lipids was determined using the method of TBARS. The method of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TBARS; Carbonyl; Salami; Ocimum basilicum L; Natural antioxidant.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200024
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Atividade antimicrobiana e antioxidante do óleo essencial de ho-sho (Cinnamomum camphora Ness e Eberm Var. Linaloolifera fujita) Ciênc. Tecnol. Aliment.
Cansian,Rogério Luis; Mossi,Altemir José; Oliveira,Débora de; Toniazzo,Geciane; Treichel,Helen; Paroul,Natalia; Astolfi,Viviane; Serafini,Luciana Atti.
Este trabalho teve como objetivo determinar a atividade antimicrobiana e antioxidante do óleo essencial de Ho-Sho. O principal componente do óleo essencial obtido a partir de folhas da planta submetidas ao processo de hidrodestilação foi o linalol (80 a 95% m/m). O óleo essencial mostrou atividade antimicrobiana para todos os microrganismos testados, com exceção de Pseudomonas aeruginosa. A maior atividade antimicrobiana do óleo essencial sobre as bactérias testadas foi observada sobre Xanthomonas campestris (33,0 mm) e a menor sobre Yersinia enterocolitica (10,5 mm). Para a concentração inibitória mínima (CIM), observou-se que todos os microrganismos apresentaram-se susceptíveis ao óleo essencial de Ho-Sho. A variação das CIM para as bactérias...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atividade antimicrobiana; Atividade antioxidante; Cinnamomum camphora.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200014
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Chemical characterization and antimicrobial activity of essential oils of salvia L. species Ciênc. Tecnol. Aliment.
Pierozan,Morgana Karen; Pauletti,Gabriel Fernandes; Rota,Luciana; Santos,Ana Cristina Atti dos; Lerin,Lindomar Alberto; di Luccio,Marco; Mossi,Altemir José; Atti-Serafini,Luciana; Cansian,Rogério Luis; Oliveira,José Vladimir.
In this work, the essential oils of S. officinalis, S. sclarea, S. lavandulifolia and S. triloba were chemically analyzed by gas chromatography coupled to a mass spectrometry detector (GC/MSD), and their antimicrobial activity was tested against 10 microorganisms using the disk diffusion method and the Minimum Inhibitory Concentration (MIC) technique. The following major compounds were identified in the essential oils: α - and β-thujone, camphor and 1,8-cineole, except in S. sclarea, where linalool, linalyl acetate and α-terpineol were the major constituents. The antimicrobial activity showed significant differences (p < 0.05) only when obtained by the MIC method. Gram-positive microorganisms presented larger sensitivity for the essential oils. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salvia; Essential oils; Antimicrobial activity; Chemical characterization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000400010
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Development and characterization of light yoghurt elaborated with Bifidobacterium animalis subsp. Lactis Bb-12 and fructooligosaccharides Ciência Rural
Witschinski,Fabiele; Demartini,Débora; Kilian,Josiane; Dallago,Rogério Marcos; Rosa,Clarissa Dalla; Cansian,Rogério Luis; Valduga,Eunice; Steffens,Juliana.
ABSTRACT: This study aimed to evaluate the effects of adding probiotic culture (Bifidobacterium animalis subsp. Lactis Bb-12) and prebiotics (fructooligosaccharide - FOS) to yoghurt formulations stored at 4°C for 28 days, using an experimental design (independent variables: (0-3% of FOS and probiotic starter cultures 0-3%). The pH, acidity, fat, syneresis, protein, ºBrix, sugars, FOS and probiotic bacteria count were analyzed. The probiotic- and prebiotic-added yoghurt formulations showed lower acidity, syneresis and glucose than the control yoghurt and compared to formulations containing probiotic and prebiotic separately. The 3% probiotic and prebiotic formulation showed a lower loss of concentration of FOS, and after 28 days presented 1.5g of FOS per...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yoghurt; Probiotic; Bifidobacterium; Prebiotics; Fructooligosaccharides.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300751
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Enzymatic hydrolysis (pepsin) assisted by ultrasound in the functional Properties of hydrolyzates from different collagens Ciência Rural
Vidal,Alessandra Roseline; Cechin,Carine da Fonseca; Cansian,Rogério Luis; Mello,Renius de Oliveira; Schmidt,Michele Mantelli; Demiate,Ivo Mottin; Kempka,Aniela Pinto; Dornelles,Rosa Cristina Prestes.
ABSTRACT: Enzymatic hydrolysis (pepsin) assisted with or without ultrasound in the functional properties of hydrolyzates from different collagens were analyzed. Degree of hydrolysis, antioxidant activity (DPPH) and antimicrobial activity (MIC) were assessed. The treatment that resulted in greater antioxidant activity for the fiber sample was with the use of 4% of enzyme and concomitant ultrasound (40.7%), leading to a degree of hydrolysis of 21.7%. For the powdered fiber sample the hydrolysis treatment with use of 4% of enzyme resulted in lower protein content (6.97mg/mL), higher degree of hydrolysis (19.9%) and greater antioxidant activity (38.6%). The hydrolyzates showed inhibitory capacity against gram-negative bacteria Salmonella choleraesuis and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pepsin; Degree of hydrolysis; Antioxidant activity; Antimicrobial activity; Ultrasound.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300752
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