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| Xiao,Na; Tang,Yi-Tong; Li,Zhi-Shan; Cao,Rui; Wang,Rong; Zou,Jiu-Ming; Pei,Jiao. |
| Abstract This study aimed to develop a simple and rapid method to detect KRAS gene mutations for conventional clinical applications under laboratory conditions. The genotype of mutation sites was determined based on the occurrence of target bands in the corresponding lanes of the reaction tubes through polymerization-conjunction of the probes, probe purification and amplification, and agarose gel electrophoresis. Circulating DNA samples were obtained from the plasma of 72 patients with lung cancer, which were identified based on six mutation sites (G12S, G12R, G12C, G12D, G12A, and G12V) of codon 12 of the KRAS gene. The detection results were compared with direct sequencing data. The proposed detection method is characterized by simple operation, high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mutation; Circulating DNA; Polymerization-conjunction reaction; Agarose gel electrophoresis; K-ras. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400555 |
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