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Kallikrein-like amidase activity in renal ischemia and reperfusion BJMBR
Carattino,M.D.; Cueva,F.; Fonovich-de-Schroeder,T.M.; Zuccollo,A..
We assessed a kallikrein-like amidase activity probably related to the kallikrein-kinin system, as well as the participation of leukocyte infiltration in renal ischemia and reperfusion. Male C57BL/KSJmdb mice were subjected to 20 or 60 min of ischemia and to different periods of reperfusion. A control group consisted of sham-operated mice, under similar conditions, except for ischemia induction. Kallikrein-like amidase activity, Evans blue extravasation and myeloperoxidase activity were measured in kidney homogenates, previously perfused with 0.9% NaCl. Plasma creatinine concentration increased only in the 60-min ischemic group. After 20 min of ischemia and 1 or 24 h of reperfusion, no change in kallikrein-like amidase activity or Evans blue extravasation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kidney; Ischemia-reperfusion; Kallikrein; Vascular permeability; Leukocyte infiltration.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500015
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Constitutive nitric oxide synthase (cNOS) activity in Langerhans islets from streptozotocin diabetic rats BJMBR
Fonovich de Schroeder,T.M.; Carattino,M.D.; Frontera,M.; Catanzaro,O.L..
Nitric oxide synthase activity was measured in Langerhans islets isolated from control and streptozotocin diabetic rats. The activity of the enzyme was linear up to 150 µg of protein from control rats and was optimal at 0.1 µM calcium, when it was measured after 45 min of incubation at 37oC in the presence of 200 µM arginine. Specific activity of the enzyme was 25 x 10-4 nmol [3H]citrulline 45 min-1 mg protein-1. Streptozotocin diabetic rats exhibited less enzyme activity both in total pancreas homogenate and in isolated Langerhans islets when compared to control animals. Nitric oxide synthase activity measured in control and diabetic rats 15 days after the last streptozotocin injection in the second group of animals corresponded only to a constitutive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide synthase; Nitric oxide; Langerhans islets; Streptozotocin; Diabetes.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500004
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