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Elimination of turbidity interference in serum iron colorimetric assay by enzymatic proteolysis BABT
Cardoso,Leonardo M.; Andrade,Milton H. G. de; Chianca Junior,Deoclécio A.; Silva,Marcelo Eustáquio; Pedrosa,Maria Lúcia.
We describe a modification in the commercial colorimetric method for the determination of serum iron by using Ferrozine® . The modification was proposed because during the conventional procedure, turbidity observed when the serum of animals submitted to surgery was used interfered with the assay. We added to the original method, a previous treatment of the serum with proteolytic enzymes. This modification was also tested using plasma samples, although this was not recommended when the original method was used. The results demonstrated that: a) the treatment with a mixture of trypsin and chymotrypsin was effective in order to eliminate turbidity; b) there was no difference between the standard curves obtained by the conventional and the modified method for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron assay; Enzymatic proteolysis; Interference.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300019
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