|
|
| Castilho, J. G.; Rodrigues, A. C.; Carnieli, P. J. R.; Oliveira, R. N.; Silva, A. C. R.; Caporale, G. M. M.; Brandão, P. E.; Batista, H. B. C. R.. |
| This study describes the comparison of in vivo and in vitro transfection of antibodies to use as a new mechanism for antiviral therapy against rabies. The transfection was made in vitro with neuroblastoma murine cell line (Neuro‐2a) and in vivo with 21 years old mice. Both cells and mice were infected with different infectious doses (0.1; 1.0; 10 and 100 ID50) of the rabies virus. After 24 hours the infected cells and mice were transfected with polyclonal antibodies against rabies produced in equines (unpurified hyperimmune serum ‐ total antibody) using a cationic reagent, the lipofectamine 2000 previously diluted 1:20, for the negative transfection control, only minimum essential medium was used. The ability of the antibodies to neutralize the rabies... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17374 |
| |
