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| Haouari,Chiraz Chaffei; Nasraoui,Afef Hajjaji; Carrayol,Elisa; Lelendais,Maud; Ghorbel,Mohamed Habib; Gouia,Houda. |
| Glutamate metabolism and amino acid translocation were investigated in the control and cadmium stressed shoots of tomato (Solanum lycopersicum - 63/5 F1), using (15N) ammonium and (15N) glutamate tracers. Regardless of organ type, [15N] ammonium assimilation occurred via glutamine synthetase (EC 6.3.1.2), in the control and stressed plants, and it did not depend on glutamate dehydrogenase (EC 1.4.1.2). The [15N] ammonium and ammonium accumulation patterns support the role of glutamate dehydrogenase in the deamination of [15N] glutamate to provide 2-oxoglutarate and [15N] ammonium. In the presence of cadmium, excess [15N] ammonium was incorporated into asparagine, which served as an additional detoxification molecule. In the presence both of methionine... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ammonium; Glutamate dehydrogenase; Methionine sulfoximine; 15N-NH4+. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000100004 |
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