|
|
|
|
|
| Gonçalves,Daniela Dib; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Alves,Lucimara Aparecida; Freire,Roberta Lemos; Navarro,Italmar Teodorico; Santana,Maria Aparecida Zanella; Santos,Luís Roberto Alves dos; Carreira,Teresa; Vieira,Maria Luísa; Freitas,Julio Cesar de. |
| The aim of this study was to conduct a serological survey for Lyme diseases, brucellosis, leptospirosis and toxoplasmosis and identify the risk variables related to these zoonoses in humans living in the rural area of Jataizinho, state of Parana, Brazil. A total of 63 rural properties were surveyed. Additionally, 207 serum samples collected from these rural area inhabitants were tested for indirect immunofluorescence (IFI) and western blots (WB) were performed to detect Borrelia burgdorferi (sensu lato); a tamponated acidified antigen test (AAT) and 2-mercaptoethanol (2-ME) were used to detect antibodies of Brucella abortus; the microscopic agglutination test (MAT) was carried out to detect antibodies anti-Leptospira spp. and IFI was used to find... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lyme disease; Leptospirosis; Brucellosis; Toxoplasmosis; Human; Antibody. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100018 |
| |
|
| |
|
|
| Gonçalves,Daniela Dib; Carreira,Teresa; Nunes,Mónica; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Vidotto,Odilon; Freitas,Julio Cesar de; Vieira,Maria Luísa. |
| The aim of this study was to investigate the presence of DNA of Borrelia burgdorferi sensu lato (s.l.) in ticks that feed on horses used for animal traction in rural Jataizinho, Parana, Brazil. Between February and June 2008, a total of 224 ticks was collected of which 75% were identified as Dermacentor nitens and 25% as Amblyomma cajenense. To amplify B. burgdorferi s.l. DNA, the intergenic space region (ISR) between the 5S (rrf) 23S (rrl) rRNA genes was used as targets for nested-PCR. Two ticks of the D. nitens species were positive for B. burgdorferi s.l. Both species showed a fragment of 184 bp, but the sequencing revealed 99.9% homology with the B. burgdorferi sensu stricto (s.s.) strain B31. These results showed, for the first time, the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ticks; Dermacentor nitens; Borrelia burgdorferi sensu lato; Equine; Rural area. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300035 |
| |
|
|
|
