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| Lima, J. Y. O.; Achkar, S. M.; Scheffer, K. C.; Castilho, J. G.; Rodrigues, A. C.; Kotait, I.; Carrieri, M. L.. |
| Bats are considered important reservoirs of rabies virus, which is paramount in the study of the pathogenesis of this zoonosis disease, through the use of sensitive systems. The presence of viral antigen in these species shows that non-neuronal viral spread is efficient in different organs that participate effectively in the elimination of rabies virus, such as, salivary glands and bladder. This study aimed to investigate the presence of rabies virus in samples of bats submitted for laboratory diagnosis, as well as, to study the pathogenesis of the disease through the use of laboratory animals. 3,930 routine diagnostic specimens of bats were processed during the period between January 2011 and May 2012 by direct immunofluorescence (DIF) and viral isolation... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3078 |
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| Scheffer, K. C.; Fahl, W. O.; Iamamoto, K.; Carnieli Jr., P.; Carrieri, M. L.; Oliveira, R. N.; Ito, F. H.. |
| Molecular techniques have been used increasingly as tools for the diagnosis by detecting the rabies virus genome. This study aimed to detect the presence of rabies virus in the wash of skull and in different organs of the genus Artibeus bats using the hemi-nested RT-PCR (hnRT-PCR) and Real Time RT-PCR molecular techniques. From approximately 4,000 specimens of bats received at the Institute Pasteur for rabies diagnosis, 30 bats of the genus Artibeus were selected, with records of positive results for rabies by the traditional techniques of direct fluorescent antibody test (FAT) and inoculation of murine neuroblastoma cell line (N2A). Salivary glands, urinary bladders, kidneys, lungs, and also the washes of the skullcaps of the specimens were collected. The... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3105 |
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| Carnieli Jr., P.; Batista, H. B.C.R.; Schereffer, K. C.; Fahl, W. O.; Lima, J. Y.O.; Oliveira, R. N.; Castilho, J. G.; Iamamoto, K.; Carrieri, M. L.; Kotait, I.. |
| The identification of animal species that transmit pathogens such as the rabies virus is of the utmost importance for public health and the natural history of infectious and contagious diseases. Diagnostic laboratories very often receive mauled or decomposing animal carcasses, particularly of bats, rendering morphometric identification unviable. The existence of different regional names for the same animal, morphological variability and the lack of staff trained in zoological identification constitute a serious problem for epidemiological surveillance. Molecular techniques are used routinely and effectively in systematics, evolution and ecology to identify species and can even be used to identify hybrids that originated from genetically close animals, in... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3106 |
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| Carrieri, M. L.; Achkar, S. M.; Castilho, J. G.; Oliveira, R. N.; Carnieli Jr, P.; Macedo, C. I.; Omoto, T. M.; Takaoka, N. Y.; Kotait, I.. |
| The São Paulo State Rabies Control Program, coordinated by the Pasteur Institute of São Paulo, has been involved in endeavors to improve the epidemiological surveillance of rabies in dogs, cats and bats, particularly in urban areas. Since 1998, when the last case of canine rabies due to antigenic variant 2 occurred in the state, the Institute has received 66,352 canine central nervous system samples, 17,796 samples from cats and 38,133 from bat specimens. Using conventional techniques for rabies diagnosis, 11 dogs, 8 cats and 554 bats were found to be positive for this disease. When molecular techniques, such as antigenic typing and genetic sequencing, were used, antigenic variant 3, which is associated with Desmodus rotundus, was detected in 11 dogs and 7... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2566 |
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| Castilho, J. G.; Batista, H. B. C. R.; Rodrigues, A. C.; Carnieli Jr, P.; Oliveira, R. N.; Silva, A. C. R.; Caporale, G. M. M.; Carrieri, M. L.; Kotait, I.; Brandão, P. E.. |
| The aim of this study was to develop a new mechanism for antiviral therapy against rabies based on the introduction by transfection with a cationic reagent (lipofectamine 2000) of antibodies into neuronal cells infected with the rabies virus. N2A cells were infected using 96-well plates and different viral concentrations (0.1, 1.0, 10 and 100 TCID50) of three lineages of rabies virus circulating in Brazil (dog, Desmodus rotundus and Eptesicus furinalis). After incubation for 24 h, the cells were transfected with antirabies- virus polyclonal antibodies and lipofectamine 2000. These cells made up the treatment group (TG). The cells in the negative control group (CG) were treated with only Minimum Essential Medium. After 11 hours, the plates were fixed with... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3080 |
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| Mori, E.; Scheffer, K. C.; Fahl, W. O.; Oliveira, R. N.; Asano, K. M.; Carnieli Jr, P.; Carrieri, M. L.; Kotait, I.. |
| Human rabies is still an important public health problem in some Brazilian regions. Usually, ante-mortem diagnosis of rabies is made by demonstration of virus antigen by direct immunofluorescence in corneal or conjunctival smears and skin biopsies; however, this technique has a low sensitivity. Recently, molecular techniques such as the reverse transcriptase polymerase chain reaction (RT-PCR) and nucleic acid sequence based amplification assay (NASBA) have been developed to improve the sensitivity and specificity of ante as well as post-mortem diagnosis of rabies. Rapid and accurate diagnosis of ante-mortem human rabies is essential for effective medical management and to ensure appropriate post-exposure prophylaxis of potential contacts with the patient.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3098 |
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| Scheffer, K. C.; Fahl, W. O.; Lamamoto, K.; Carnieli Junior, P.; Carrieri, M. L.; Mori, E.; Oliveira, R. N.; Ito, F. H.. |
| The aim of this study was to detect the rabies virus (RABV) presence in different organs of frugivorous bats using molecular techniques such as RT‐PCR, hnRT‐PCR, and the Real Time RT‐PCR. Thirty bats of the genus Artibeus were selected and resulted as positive by the DFA test and N2A‐cells inoculation test using brain tissue in both tests. Samples of salivary gland tissue, urinary bladder tissue, kidney tissue, lung tissue, stool, and skull lavage were collected for molecular assays. The organs and the stool were diluted at 1:10 (w/v) and the urinary bladder was diluted at 1:20 (w/v). The RT‐PCR and the hnRT‐PCR were performed using specific nucleoprotein gene‐target primers. The product obtained by reverse transcription technique was submitted to the Real... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17385 |
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| Lima, J. Y. O.; Scheffer, K. C.; Achkar, S. M.; Kotait, I.; Carrieri, M. L.. |
| The diversity of bat species in Brazil is great, and there are 172 species distributed among nine families. The Phyllostomidae family is the most numerous, followed by Vespertilionidae and Molossidae. According to feeding habits, the majority of bats are insectivorous, followed by frugivorous, nectarivorous, carnivorous and hematophagous. As these animals are considered reservoirs of rabies virus it is essential to correctly identify the species and knowledge of the c rmv s p . g o v . b r mv&z 79 biology, behavior and adaptation of bats in urban environments. The objective of this study was to identify the several specimens of bats received for rabies diagnosis at the Pasteur Institute, meet the positive index of them and assess their distribution on... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3079 |
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