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| Salem,Julia Ignez; Carvalho,Clarice Maia; Ogusku,Mauricio Morishi; Maia,Rosália; Ruffino-Netto,Antonio. |
| We elaborated an alternative culture method, which we denominated PKO (initials in tribute of respect to Petroff, Kudoh and Ogawa), for isolating Mycobacterium tuberculosis from sputum for diagnosis of pulmonary tuberculosis (TB), and to compare its performance with the Swab and Petroff methods. For the technique validation, sputum samples from patients suspected of pulmonary TB cases were examined by acid-fast microscopy (direct and concentrated smear), PKO, Swab and Petroff methods. We found that Petroff and PKO methods have parity in the effectiveness of M. tuberculosis isolation. However, by the PKO method, 65% of isolated strains were detected in a period of <FONT FACE=Symbol>£</FONT>15 days, while by the Petroff method the best detection... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnostic method; Mycobacterium tuberculosis; PKO; Tuberculosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000300013 |
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| Lima,Alita Moura de; Salem,Júlia Ignez; Souza,João Vicente Braga de; Cortez,Ana Claudia Alves; Carvalho,Clarice Maia; Chaves,Francisco Célio Maia; Veiga Junior,Valdir Florêncio da. |
| Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from... |
| Tipo: Journal article |
Palavras-chave: Antagonism; Stimulation growth; Tuberculosis. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400008 |
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