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Carvalho,S.A.; Carmo,L.S.; Abreu,E.F.; Dias,R.S.; Apolônio,A.C.M.; Santos,S.G.; Farias,L.M.; Carvalho,M.A.R.. |
The production of Toxic Shock Syndrome Toxin-1 (TSST-1), enterotoxins and bacteriocin-like substances was evaluated in 95 strains of Staphylococcus aureus recovered from raw bovine milk (n=31) and from food samples involved in staphylococcal food poisoning (n=64). Enterotoxigenicity tests with the membrane over agar associated to optimal sensibility plate assays were performed and showed that 96.77% of strains recovered from milk and 95.31% from food samples produced enterotoxins A, B, C, D or TSST-1. Reference strains S. epidermidis, Bacillus cereus, Listeria monocytogenes, Lactobacillus casei, Pseudomonas aeruginosa, S. aureus, Salmonella Typhimurium, Escherichia coli, Enterococcus faecalis and Bacteroides fragilis were used as indicator bacteria in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Mastitis; Toxigenicity; Food poisoning; Bacteriocin. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500035 |
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Lafetá,B.N.; Santos,S.; Silva,V.L.; Carvalho,M.A.R.; Diniz,C.G.; Silva,N.. |
Estudou-se o perfil das proteínas da membrana externa (PME) da Leptospira interrogans sorovariedade Hardjoprajitno por meio da eletroforese bidimensional. Foram utilizadas técnicas de extração das PME com Triton x114 e precipitação com acetona. Os géis foram corados com nitrato de prata e as imagens analisadas para determinação da massa molecular das proteínas detectadas. Foram visualizadas 35 bandas protéicas, sendo que cinco delas se destacaram por estarem em maior quantidade: 22,54KDa (LipL22), 30/26KDa (LipL32), 34,41KDa (PME34), 42,75KDa (LipL41) e 58,59KDa (LipL63). |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira interrogans Hardjoprajitno; Proteínas de membrana; Eletroforese bidimensional. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600002 |
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Carneiro,L.P.; Rezende,C.M.F.; Silva,C.A.; Laranjeira,M.G.; Carvalho,M.A.R.; Farias,L.M.. |
Eleven dogs, of both sexes, average weight of 12kg, with ages ranging from two to five years were used with the objective of evaluating two treatments of skin wounds produced after percutaneous bone transfixation of the tibia. In six animals, cotton swabs embedded in 0.2% iodine-alcohol were used to clean the areas of pin perforations. In five dogs, a 0.9% sodium chloride solution, under approximate 30 psi pulse pressure, was used. In both cases, and treatments were carried out every three to four days, during four weeks. Clinical specimens were collected under anaerobic conditions, immediately before the treatments, and on days 3, 7, 14 and 28 after surgery. Samples were cultured in specific media in order to allow isolation of aerobic and anaerobic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; External skeletal fixation; Post-surgical complication. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400008 |
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Branco,K.M.G.R.; Nardi,R.M.D.; Moreira,J.L.S.; Nunes,A.C.; Farias,L.M.; Nicoli,J.R.; Carvalho,M.A.R.. |
Lactobacilli isolated from the vaginal tract of women with and without bacterial vaginosis (BV) were identified and characterized for the production of antagonists. Bacterial samples were isolated from healthy women (N = 16), from patients with clinical complaints but without BV (N = 30), and from patients with BV (N = 32). Identification was performed using amplified ribosomal DNA restriction analysis. Production of antagonistic compounds was evaluated by the double-layer diffusion technique using Gram-positive (N = 9) and Gram-negative bacteria (N = 6) as well as yeast (N = 5) as indicator strains. Of a total of 147 isolates, 133 were identified as pertaining to the genus Lactobacillus. Lactobacillus crispatus was the species most frequently recovered,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactobacillus; Bacterial vaginosis; Antagonism; Hydrogen peroxide; Identification. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400003 |
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