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Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil BJID
Rodrigues,Ana Claudia Souza; Chang,Marilene Rodrigues; Nóbrega,Gabriela Dorn; Rodrigues,Mayara Souza; Carvalho,Nadia Cristina Pereira; Gomes,Beatriz Garcia; Almeida,Dênio Lopes de; Carvalho,Karyne Rangel; Asensi,Marise Dutra.
Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa drug resistance; Multiple beta-lactamases electrophoresis gel pulsed-field.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300002
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