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	Provedor de dados BJM (2) Rev. Microbiol. (1) Autor Carvalho,Patrícia de Oliveira (3) Contesini,Fabiano Jares (2) Alberto,Thiago Grando (1) Araújo,Maria Elisa Melo Branco de (1) Campos,Paula Renata Bueno (1) Ikegaki,Masaharu (1) Oliveira,Joaquim Gilberto de (1) Pastore,Gláucia Maria (1) Mais... Palavra-chave Gamma-linolenic acid (1) Immobilization (1) Lipase (1) Lipase (R (1) Mucor sp (1) Polyunsaturated fatty acids (1) S)-ibuprofen (R (1) S)-ketoprofen enantioselectivity (1) Soybean oil (1) Unsaturated fatty acids (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2016 (1) 2006 (1) 1999 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Enhancement of gamma-linolenic acid production by the fungus Mucor sp LB-54 by growth temperature Rev. Microbiol. Carvalho,Patrícia de Oliveira; Oliveira,Joaquim Gilberto de; Pastore,Gláucia Maria. As a relatively prolific producer of GLA, the strain of Mucor sp LB-54 was selected for a study at different growth temperatures in shaker flask culture. The strain used in our experiment was capable to accumulate a relatively high amount of intracellular lipid, 20.73 % of dry cell weight, and GLA content of 15 % of total fatty acids after 5 days of incubation at 28°C. As the growth temperature was decreased from 28 to 12°C the percentage of GLA increased from 15 to 24 % of total fatty acids. In order to optimize the culture conditions for rapid biomass production and lipid production with a high proportion of GLA, the fungus was grown at two temperature combinations associated supplies of carbon source (glucose) in the culture medium. Maximal production... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma-linolenic acid; Mucor sp; Unsaturated fatty acids. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200016 Enzymatic resolution of (R,S)-ibuprofen and (R,S)-ketoprofen by microbial lipases from native and commercial sources BJM Carvalho,Patrícia de Oliveira; Contesini,Fabiano Jares; Ikegaki,Masaharu. The enantioselectivity (E) of native lipases from Aspergillus niger, Aspergillus terreus, Fusarium oxysporum, Mucor javanicus, Penicillium solitum and Rhizopus javanicus in the resolution of (R,S)-ibuprofen and (R,S)-ketoprofenenantiomers by esterification reaction with 1-propanol in isooctane was compared with known commercial Candida rugosa (Sigma) and Candida antarctica (Novozym®435) lipases. In the resolution of (R,S)-ibuprofen, C. rugosa lipase showed good selectivity (E = 12) while Novozym®435 (E = 6.7) and A. niger (E = 4.8) lipases had intermediate selectivities. Other enzymes were much less selective (E around 2.3 and 1.5), under tested conditions. After preliminary optimization of reaction conditions (water content, enzyme concentration and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase (R; S)-ibuprofen (R; S)-ketoprofen enantioselectivity. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300024 Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase BJM Araújo,Maria Elisa Melo Branco de; Campos,Paula Renata Bueno; Alberto,Thiago Grando; Contesini,Fabiano Jares; Carvalho,Patrícia de Oliveira. Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4 °C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Immobilization; Soybean oil; Polyunsaturated fatty acids. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401006 Registros recuperados: 3 Primeira ... 1 ... Última

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