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PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO BJM
Carvalho,Rosangela Nunes; Oliveira,Antonio Nonato de; Mesquita,Albenones José de; Rezende,Cíntia Silva Minafra e; Mesquita,Adriano Queiroz de; Romero,Rolando Alfredo Mazzoni.
Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minas Frescal cheese; STEC/VTEC; PCR; VIDAS ECO O157®.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100002
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Application of extruded broken bean flour for formulation of gluten-free cake blends Ciênc. Tecnol. Aliment.
Gomes,Luciana de Oliveira Froes; Santiago,Raquel de Andrade Cardoso; Carvalho,Ana Vania; Carvalho,Rosangela Nunes; Oliveira,Iana Gabriela de; Bassinello,Priscila Zaczuk.
The aim of this study was to determine the physical and microbiological characteristics of extruded broken beans flour, in addition to developing mixtures for gluten-free cake with these flours, evaluating their technological and sensory quality. Gluten-free formulations were prepared with 45%, 60% and 75% of extruded broken beans. All analyzes of the flours and mixtures for cakes were performed according to standard techniques found in the literature. Sensory analyzes of cakes applied the 9-point structured hedonic scale. Results were submitted to variance analysis and comparison of means test (Tukey, p<0.05). The use of extruded broken beans improved the water absorbed and water solubility index of the mixtures for gluten-free cake, and for the lower...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Byproducts; Technological characteristics; Acceptability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000200307
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Processing and characterization of extruded breakfast meal formulated with broken rice and bean flour Ciênc. Tecnol. Aliment.
Carvalho,Ana Vânia; Mattietto,Rafaella de Andrade; Bassinello,Priscila Zaczuk; Koakuzu,Selma Nakamoto; Rios,Alessandro de Oliveira; Maciel,Renan de Almeida; Carvalho,Rosangela Nunes.
The objective of this work was to develop an extruded breakfast product containing broken rice and split old beans and to verify the influence of the extrusion process on their physicochemical, technological, and sensory characteristic. The final product had a protein content of 9.9 g.100 g-1, and therefore it can be considered a good source of proteins for children and teenagers. The dietary fiber content of the final edible product was 3.71 g.100 g-1. Therefore, the breakfast meal may be considered as a source of dietary fiber according to Brazilian law . As for the technological properties, the extruded product presented an expansion index of 8.89 and apparent density of 0.25 g.cm-3. With regard to the sensory analysis, the acceptance average was ranked...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Oryza sativa L.; Thermoplastic extrusion; Technological properties; Nutritional characteristics; Sensory acceptability.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300014
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