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| Burbano,R.R.; Medeiros,A.C.; Mello,A.A.; Lemos,J.A.; Bahia,M.O.; Casartelli,C.. |
| Breast cancer in families with germ line mutations in the TP53 gene has been described in the medical literature. Mutation screening for susceptibility genes should allow effective prophylactic and preventive measures. Using single-strand conformational polymorphism, we screened for mutations in exons 5, 6, 7 and 8 of gene TP53 in the peripheral blood of 8 young non-affected members (17 to 36 years old) of families with a history of breast cancer. Studies of this type on young patients (mean age, 25 years) are very rare in the literature. The identification of these mutations would contribute to genetic counseling of members of families with predisposition to breast cancer. The results obtained did not show any polymorphism indicating mutation. In our... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: TP53; Family history of breast cancer; Mutation screening; Early breast cancer. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100010 |
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| Lima,E.M.; Rissino,J.D.; Harada,M.L.; Assumpção,P.P.; Demachki,S.; Guimarães,A.C.; Casartelli,C.; Smith,M.A.C.; Burbano,R.R.. |
| Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell line ACP01; Human gastric adenocarcinoma; In vitro immortalization; Chromosome 8 trisomy; Clonal expansion. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200008 |
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| Lima,E.M.; Leal,M.F.; Burbano,R.R.; Khayat,A.S.; Assumpção,P.P.; Bello,M.J.; Rey,J.A.; Smith,M.A.C.; Casartelli,C.. |
| Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA methylation; Gastric cancer; ANAPC1; CDKN2A; TP53. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600017 |
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| Burbano,R.R.; Lima,E.M.; Khayat,A.S.; Barbieri Neto,J.; Cabral,I.R.; Bastos Jr.,L.; Bahia,M.O.; Casartelli,C.. |
| Twelve breast fibroadenomas were analyzed cytogenetically and only four were found to have clonal alterations. The presence of chromosomal alterations in fibroadenomas must be the consequence of the proliferating process and must not be related to the etiology of this type of lesion. In contrast, the few fibroadenomas that exhibit chromosomal alterations are likely to be those presenting a risk of neoplastic transformation. Clonal numerical alterations involved chromosomes 8, 18, 19, and 21. Of the chromosomal alterations found in the present study, only monosomy of chromosomes 19 and 21 has been reported in breast fibroadenomas. The loss of chromosome 21 was the most frequent alteration found in our sample. The study of benign proliferations and their... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Fibroadenomas; Chromosome alterations; Breast cancer. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800005 |
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