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| Casseb,J.; Katzenstein,D.; Winters,M.; Brigido,L.F.M.; Duarte,A.J.S.; Hendry,R.M.. |
| The main objective of the present study was to assess the specificity and sensitivity of a modified assay using short synthetic peptides of the V3 region of HIV-1 gp120, which is the main target for neutralizing antibodies. Results from an enzyme immunoassay (EIA) employing a panel of synthetic peptides of HIV-1 subtypes and using urea washes to detect high avidity antibodies (AAV3) were compared with those obtained by the heteroduplex mobility assay and DNA sequencing. The EIA correctly typed 100% of subtype B (sensitivity = 1.0; specificity = 0.95), 100% of HIV-1 E samples (sensitivity = 1.0; specificity = 1.0), and 95% of subtype C specimens (sensitivity = 0.95; specificity = 0.94). In contrast, only 50% of subtype A (sensitivity = 0.5; specificity =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Viral diversity; Serotypes; V3 serology. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300013 |
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| Casseb,J.; Hong,M.A.; Gonsalez,C.; Brígido,L.F.; Duarte,A.J.S.; Michael-Hendry,R.. |
| HIV-1 variability may have an important impact on transmission and pathogenicity. Better characterization of the HIV epidemic in Brazil is necessary for the development of vaccine trials in this country. We analyzed sera from 108 HIV-1-infected volunteers from São Paulo City to determine serotype and reactivity for V3 motifs of HIV in this population, and the relationship to transmission mode. We concluded that the HIV-1 B serotype is frequent among heterosexually infected women, even in the absence of anal sex, and that two major V3 motifs, GPGR and GWGR, had similar prevalence among women (48% and 52%, respectively) and men (56% and 44%, respectively). We also observed an equal distribution of these strains regardless of their CD4+ T cell counts,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HIV; Serotyping; Gp 120 V3 loop; Synthetic peptides; Sexual transmission. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000002 |
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| Komninakis,S.; Fukumori,L.; Alcalde,R.; Cortina,M.; Abdala,L.; Brito,A.; Sanabani,S.; Duarte,A.J.S.; Casseb,J.. |
| The purpose of the present study was to compare the sensitivity and specificity of V3 enzyme immunoassay (solid phase EIA and EIA inhibition) and restriction fragment length polymorphism (RFLP) with the DNA sequencing "gold standard" to identify the Brazilian HIV-1 variants of subtype B and B"-GWGR. Peripheral blood mononuclear cells were collected from 61 HIV-1-infected individuals attending a clinic in São Paulo. Proviral DNA was amplified and sequentially cleaved with the Fok I restriction enzyme. Plasma samples were submitted to a V3-loop biotinylated synthetic peptide EIA. Direct partial DNA sequencing of the env gene was performed on all samples. Based on EIA results, the sensitivity for detecting B-GPGR was 70%, compared to 64% for the Brazilian... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1 subtyping; Brazilian subtype B variant; Serotyping; DNA sequencing. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300003 |
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| Keller,R.; Zago,A.; Viana,M.C.; Bourboulia,D.; Desgranges,C.; Casseb,J.; Moura,W.V.; Dietze,R.; Collandre,H.. |
| The aims of the present study were to determine the prevalence of human herpesvirus type 8 (HHV-8) in HIV-positive Brazilian patients with (HIV+/KS+) and without Kaposi's sarcoma (HIV+/KS-) using PCR and immunofluorescence assays, to assess its association with KS disease, to evaluate the performance of these tests in detecting HHV-8 infection, and to investigate the association between anti-HHV-8 antibody titers, CD4 counts and staging of KS disease. Blood samples from 66 patients, 39 HIV+/KS+ and 27 HIV+/KS-, were analyzed for HHV-8 viremia in peripheral blood mononuclear cells by PCR and HHV-8 antigenemia for latent and lytic infection by immunofluorescence assay. Positive samples for latent nuclear HHV-8 antigen (LNA) antibodies were titrated out from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AIDS-related Kaposi's sarcoma; Human herpesvirus type 8; HHV-8; Brazil. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700007 |
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| Montanheiro,P.A.; Penalva de Oliveira,A.C.; Posada-Vergara,M.P.; Milagres,A.C.; Tauil,C.; Marchiori,P.E.; Duarte,A.J.S.; Casseb,J.. |
| To evaluate the human T-cell lymphotropic virus type I (HTLV-I) proviral DNA load among asymptomatic HTLV-I-infected carriers and patients with HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP), real time PCR using TaqMan probes for the pol gene was performed in two million peripheral blood mononuclear cells (PBMC). The albumin gene was the internal genomic control and MT2 cells were used as positive control. The results are reported as copies/10,000 PBMC, and the detection limit was 10 copies. A total of 89 subjects (44 HAM/TSP and 45 healthy HTLV-I-infected carriers) followed up at the Institute of Infectious Diseases "Emilio Ribas" and in the Neurology Division of Hospital of Clínicas were studied. The asymptomatic HTLV-I-infected... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HTLV-I; HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP); HTLV-I DNA viral load. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100011 |
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