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	Provedor de dados BABT (2) Autor Castilho,Leda dos Reis (2) Cavalcanti,Elisa d'Avila Costa (1) Freire,Denise Maria Guimarães (1) Freire,Marcos da Silva (1) Gutarra,Melissa Limoeiro Estrada (1) Sant'Anna Júnior,Geraldo Lippel (1) Souza,Marta Cristina de Oliveira (1) Mais... Palavra-chave Cytodex 1 (1) Cytodex 3 (1) Fixed-bed bioreactors (1) High-cell-density cultivation (1) Lipase (1) Microcarriers (1) Penicillium simplicissimum (1) Solid-state fermentation (1) Vero cells (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Lipase production by solid-state fermentation in fixed-bed bioreactors BABT Cavalcanti,Elisa d'Avila Costa; Gutarra,Melissa Limoeiro Estrada; Freire,Denise Maria Guimarães; Castilho,Leda dos Reis; Sant'Anna Júnior,Geraldo Lippel. In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min. Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state fermentation; Fixed-bed bioreactors; Lipase; Penicillium simplicissimum. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400010 Influence of culture conditions on Vero cell propagation on non-porous microcarriers BABT Souza,Marta Cristina de Oliveira; Freire,Marcos da Silva; Castilho,Leda dos Reis. Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are being increasingly used. The use of microcarriers in stirred bioreactors allows high cell densities and, consequently, high virus titres to be achieved. With the aim of selecting appropriate culture conditions for the cultivation of Vero cells at high cell densities, in this work the influence of several variables (agitation rate, ratio of inoculated cells to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Vero cells; Microcarriers; Cytodex 1; Cytodex 3; High-cell-density cultivation. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400009 Registros recuperados: 2 Primeira ... 1 ... Última

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