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Leptospira spp detection by Polymerase Chain Reaction (PCR) in clinical samples of captive black-capped Capuchin monkey (Cebus apella) BJM
Scarcelli,Eliana; Piatti,Rosa Maria; Fedullo,José Daniel Luzes; Simon,Faiçal; Cardoso,Maristela Vasconcellos; Castro,Vanessa; Miyashiro,Simone; Genovez,Margareth Élide.
Leptospirosis is a widely distributed zoonosis that affects domestic and wild animals, and that has the man as the end point of its epidemiological chain. Leptospirosis diagnosis in primates is more difficult than in other animal species, as clinical signs and lesions are less evident and antibody response is detected only for short periods. The aim of this article was to describe the detection of Leptospira spp using polymerase chain reaction (PCR), in clinical samples from one captive black-capped Capuchin monkey (Cebus apella), which presented characteristics compatible with leptospirosis (jaundice and haemorrhagic kdney) in the macroscopic post-mortem examination. A friable kidney fragment and urine sample were cultured and submitted to experimental...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Nonhuman primate; PCR; Cebus apella.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200010
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Interference of vaccinal antibodies on serological diagnosis of leptospirosis in vaccinated buffalo using two types of commercial vaccines BJM
Nardi Júnior,Geraldo de; Genovez,Margareth Elide; Ribeiro,Marcio Garcia; Castro,Vanessa; Jorge,André Mendes.
The vaccinal antibodies interference represents one of the Microscopic Agglutination test - MAT limitation in the animal leptospirosis serum diagnosis. Prospective studies showing the dimensions of this effect are rare in buffaloes. This study aimed to determine the anti-Leptospira serum agglutinin profile in vaccinated female buffaloes using two types of commercial vaccines against leptospirosis: bacterin (whole bacterial cell) and purified outer membrane and to evaluate the vaccinal interference on serum diagnosis. Three groups of 11 adult buffalo females were established: G1-control, non-vaccinated, G2- vaccinated with bacterin vaccine with six serovars, G3- outer membrane purified vaccine with five serovars. A booster dose was administrated 30 days...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo leptospirosis; Vaccination; Serum diagnosis; MAT.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200033
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Seroprevalence and risk factors of antibodies against Leptospira spp. in ovines from Uberlândia municipality, Minas Gerais state, Brazil BJM
Salaberry,Sandra R.S.; Castro,Vanessa; Nassar,Alessandra F.C.; Castro,Jacqueline R.; Guimarães,Ednaldo C.; Lima-Ribeiro,Anna M.C..
The objectives of the present study were to verify the seroprevalence of anti-Leptospira spp. antibodies, identify the most frequent serovars and the risk factors associated with the infection in Uberlândia, Minas Gerais, Brazil . A total of 334 ovines blood samples were collected in 12 farms from Uberlândia municipality to be evaluated by means the Microscopic Agglutination Test (MAT) against 22 serovars of Leptospira spp. and an epidemiologic questionnaire was applied for each farm in order to correlate with risk factors of leptospirosis: sex, age and breed as well as contact with cattle, contact with dogs and presence of rodents. The prevalence of seropositive to MAT was found in seventy four ovines (22.2%; CI 95% 17.6-26.4%), with titers ranging from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Seroprevalence; Serovars; Sheep.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400026
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Frequency of and risk factors associated to Leptospira spp. Seropositivity in goats in the state of Sergipe, Northeastern Brazil Ciência Rural
Rizzo,Huber; Silva,Tatiane Rodrigues da; Carvalho,Jeferson Silva; Marinho,Felipe Apolônio; Santos,Hilderley de Almeida; Silva Júnior,Wellington Santos; Alemán,Mario Augusto Reyes; Pinheiro Júnior,José Wilton; Castro,Vanessa.
ABSTRACT: The objective of this study was to determine the frequency and factors associated with the occurrence of seropositivity for Leptospira spp. in goat herds in the state of Sergipe, Brazil, on the basis of the analysis of 675 samples collected from 41 properties in 2013-2014. Analysis of anti-Leptospira spp. antibodies revealed that 25.74% goats (194) were seropositive and that 90.24% (37) of the properties had at least one infected goat. The most prevalent serogroup was Icterohaemorrhagiae (85.57%); although, the study animals were reactive to other serogroups: Australis (5.15%), Pomona (3.61%), Sejroe (3.09%), and Pyrogenes (2.58%) had titers ≤400 in 96.91% of cases. The origin of the water source [still water source (OR=3.86)] was associated with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water source; Leptospirosis; Serogroup; Icterohaemorrhagiae; Test microscopic agglutination test.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700452
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