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The use of singleplex and nested PCR to detect Batrachochytrium dendrobatidis in free-living frogs BJM
Coutinho,Selene Dall'Acqua; Burke,Julieta Catarina; Paula,Catia Dejuste de; Rodrigues,Miguel Trefaut; Catão-Dias,José Luiz.
Many microorganisms are able to cause diseases in amphibians, and in the past few years one of the most reported has been Batrachochytrium dendrobatidis. This fungus was first reported in Brazil in 2005; following this, other reports were made in specimens deposited in museum collections, captive and free-living frogs. The aim of this study was to compare singleplex and nested-PCR techniques to detect B. dendrobatidis in free-living and apparently healthy adult frogs from the Brazilian Atlantic Forest. The sample collection area was a protected government park, with no general entrance permitted and no management of the animals there. Swabs were taken from the skin of 107 animals without macroscopic lesions and they were maintained in ethanol p.a. Fungal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chytridiomycosis; Batrachochytrium dendrobatidis; Frogs; Brazilian Atlantic Forest; PCR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200551
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Detection of Mycobacterium avium in pet birds BJM
Godoy,Silvia Neri; Sakamoto,Sidnei Miyoshi; Paula,Cátia Dejuste de; Catão-Dias,José Luiz; Matushima,Eliana Reiko.
The present study is a report on the presence of Mycobacterium avium in four birds of the psittaciform order kept as pets. Anatomopathological diagnosis showed lesions suggestive of the agent and presence of alcohol-acid resistant bacilli (AARB) shown by the Ziehl-Neelsen staining. The identification of Mycobacterium avium was performed by means of PRA (PCR Restriction Analysis). DNA was directly extracted from tissue of the lesions and blocked in paraffin. The role of this agent in pet bird infection is discussed, as well as its zoonotic potential.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium avium; Psittacines; Pathology; Birds.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200011
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