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Identification of Candida spp. by phenotypic tests and PCR BJM
Marinho,Sandra Aparecida; Teixeira,Alice Becker; Santos,Otávio Silveira; Cazanova,Ricardo Flores; Ferreira,Carlos Alexandre Sanchez; Cherubini,Karen; Oliveira,Sílvia Dias de.
The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance, allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production, culture in CHROMAgarTM Candida, carbohydrate assimilation test, growth at 45ºC and culture in Tween 80 agar. Genotypic confirmation was performed by PCR. Phenotypic tests showed that 63.2% strains formed germ-tubes, 73.7% produced chlamydoconidia, and 63.2% showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida spp; Identification; PCR; Phenotypic tests.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200004
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