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	Provedor de dados 65 (2) Autor Ceccon,Cássia Canzi (2) Grando,Magali Ferrari (2) Almeida,Vinícius de Oliveira (1) Caverzan,Andréia (1) Didoné,Dielli Aparecida (1) Margis,Rogerio (1) Salvadori,José Roberto (1) Silva,Marilia Rodrigues de (1) Mais... Palavra-chave Co-transformation (1) EHA 101 (1) In vitro regeneration (1) Minichromosomes. (1) PTF102 (1) Re-transformation (1) Sexual crossing (1) Temperature stress (1) Transgene (1) Zea mays (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2020 (1) 2017 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Gene stacking as a strategy to confer characteristics of agronomic importance in plants by genetic engineering 65 Ceccon,Cássia Canzi; Caverzan,Andréia; Margis,Rogerio; Salvadori,José Roberto; Grando,Magali Ferrari. ABSTRACT: Gene stacking refers to the introduction of two or more transgenes of agronomic interest in the same plant. The main methods for genetically engineering plants with gene stacking involve (i) the simultaneous introduction, by the co-transformation process, and (ii) the sequential introduction of genes using the re-transformation processes or the sexual crossing between separate transgenic events. In general, the choice of the best method varies according to the species of interest and the availability of genetic constructions and preexisting transgenic events. We also present here the use of minichromosome technology as a potential future gene stacking technology. The purpose of this review was to discuss aspects related to the methodology for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-transformation; Re-transformation; Sexual crossing; Transgene; Minichromosomes.. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000600401 Genetic transformation of the Brazilian BR 451 maize variety by the Agrobacterium tumefaciens method 65 Silva,Marilia Rodrigues de; Didoné,Dielli Aparecida; Ceccon,Cássia Canzi; Almeida,Vinícius de Oliveira; Grando,Magali Ferrari. ABSTRACT: The asexually gene introduction by genetic engineering has brought enormous possibilities to innovate plant breeding. However, principally because of the low in vitro response, genetic transformation has been restricted to only certain genotypes of agronomically significant species. With the objective of establishing a protocol for genetically transforming the Brazilian BR 451 maize variety through Agrobacterium tumefaciens, it was studied the capacity of plant regeneration in vitro from embryogenic calli cultivated in three regeneration media, each having different growth regulators. It was also evaluated the temperature stress effect on the transformation of the immature embryos with A. tumefaciens EHA 101 containing the plasmid pTF102 with... Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro regeneration; Temperature stress; EHA 101; PTF102; Zea mays. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100151 Registros recuperados: 2 Primeira ... 1 ... Última

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