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	Provedor de dados BJMBR (2) Autor Cedenho,A.P. (2) Avellar,M.C.W. (1) Esteves,S.C. (1) Fraietta,R. (1) Porto,C.S. (1) Spaine,D. (1) Spaine,D.M. (1) Srougi,M. (1) SãoPedro,S.L. (1) Mais... Palavra-chave AZF region (1) Acrosome (1) Azoospermia (1) Azoospermia factor (1) Cryopreservation (1) Human spermatozoa (1) Ionophore (1) Male infertility (1) Microdeletions (1) Oligoasthenozoospermia (1) Pentoxifylline (1) Severe oligozoospermia (1) Y chromosome (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2007 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men BJMBR SãoPedro,S.L.; Fraietta,R.; Spaine,D.; Porto,C.S.; Srougi,M.; Cedenho,A.P.; Avellar,M.C.W.. We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified efficiently in samples from 12 fertile men tested, but failed to be amplified in samples from fertile women, indicating the specificity of PCR conditions for Yq screening. Overall, 4 of the 60 infertile patients tested (6.7%) exhibited deletion of the Y chromosome, 2 of them being severely oligozoospermic patients (P10 and P32) and 2 azoospermic men (patients P47 and P57). Patients P47 and P57 presented... Tipo: Info:eu-repo/semantics/article Palavras-chave: Y chromosome; Microdeletions; Severe oligozoospermia; Azoospermia; Male infertility; Azoospermia factor; AZF region. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600015 Effects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method BJMBR Esteves,S.C.; Spaine,D.M.; Cedenho,A.P.. The objective of the present study was to investigate the effects of the direct addition of pentoxifylline (PF) to the ejaculates of men with poor sperm quality before freezing on post-thaw sperm motility, viability, acrosome integrity, and agonist-induced acrosome reaction. Semen specimens from 16 infertile men with impaired sperm count and motility (oligoasthenozoospermia) were divided into two equal aliquots: one received no treatment (control) while the other was incubated with 5 mM PF (treated). Both aliquots were cryopreserved by the liquid nitrogen vapor method. Motility was assessed according to WHO criteria. Acrosome integrity and spontaneous and calcium ionophore-induced acrosome reactions were assessed with fluorescein isothiocyanate-conjugated... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acrosome; Cryopreservation; Human spermatozoa; Ionophore; Pentoxifylline; Oligoasthenozoospermia. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000700014 Registros recuperados: 2 Primeira ... 1 ... Última

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