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Increased expression of keratinase and other peptidases by Candida parapsilosis mutants BJMBR
Duarte,T.R.; Oliveira,S.S.; Macrae,A.; Cedrola,S.M.L.; Mazotto,A.M.; Souza,E.P.; Melo,A.C.N.; Vermelho,A.B..
Keratinases are enzymes of great importance involved in pathogenic processes of some fungi. They also have a widespread ecological role since they are responsible for the degradation and recycling of keratin. On the one hand, studying them furthers our knowledge of pathogenicity mechanisms, which has important implications for human health, and on the other hand, understanding their ecological role in keratin recycling has biotechnological potential. Here, a wild-type keratinolytic Candida parapsilosis strain isolated from a poultry farm was treated with ethyl methanesulfonate in order to generate mutants with increased keratinase activity. Mutants were then cultured on media with keratin extracted from chicken feathers as the sole source of nitrogen and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida parapsilosis; Keratinase; Ethyl methanesulfonate; Mutants.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300006
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