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	Provedor de dados Genet. Mol. Biol. (5) BABT (1) PAB (1) Autor Ceron,Carlos Roberto (7) Baffi,Milla Alves (2) Bonetti,Ana Maria (2) Carareto,Claudia Marcia Aparecida (2) Commar,Leliane Silva (2) Pereira,Cícero Donizete (2) Souza,Guilherme Rocha Lino de (2) Azeredo-Oliveira,Maria Tercilia Vilela de (1) Bicudo,Hermione E.M.C. (1) Bonilla-Rodriguez,Gustavo Orlando (1) Cabral,Hamilton (1) Galego,Luis Gustavo da Conceição (1) Gourlart,Luiz Ricardo (1) Lucca Jr.,Marcos de (1) Mateus,Rogério Pincela (1) Rahal,Paula (1) Tavares,Mara Garcia (1) Mais... Palavra-chave Esterases (2) Alloenzyme (1) Bari-I (1) Bioinvasion (1) Boophilus microplus (1) Cattle tick (1) D. navojoa (1) D. simulans (1) Drosophila arizonae (1) Drosophila melanogaster (1) Drosophila mojavensis (1) Drosophila mulleri (1) Interspecific hybrids (1) Introgressive hybridization (1) Isoenzymes (1) Molecular markers (1) Molecular weight determination (1) Nucleolar dominance (1) PAGE (1) Pesticides (1) Mais... Tipo do documento Info:eu-repo/semantics/article (6) Info:eu-repo/semantics/other (1) Ano 2012 (1) 2009 (1) 2007 (3) 2005 (1) 1998 (1) País Brazil (7) Idioma Inglês (7)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 7 Primeira ... 1 ... Última Tissue-specific expression of esterases in Triatoma infestans (Triatominae, Heteroptera) Genet. Mol. Biol. Tavares,Mara Garcia; Azeredo-Oliveira,Maria Tercilia Vilela de; Ceron,Carlos Roberto. We examined the esterases present in the hemolymph and Malpighian tubules of "Kissing bug", Triatoma infestans (Triatominae, Heteroptera) by polyacrylamide gel electrophoresis. Six esterase bands were observed and were designated EST 1 to EST 6. EST 1, 4, 5 and 6 were exclusive to hemolymph, whereas EST 2 and 3 were found only in Malpighian tubules. Each tissue had a characteristic esterase pattern, which may be related to its functional role. The four hemolymph esterases hydrolyzed a-naphthyl acetate. One of these enzymes was classified as a carboxylesterase (EST 4), and another was an acetylesterase (EST 6). The other two enzymes (EST 1 and 5) could be either carboxylesterases or serino-proteases with an esterolytic function, as they were selectively... Tipo: Info:eu-repo/semantics/article Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400009 Taxonomic and evolutionary analysis of Zaprionus indianus and its colonization of Palearctic and Neotropical regions Genet. Mol. Biol. Commar,Leliane Silva; Galego,Luis Gustavo da Conceição; Ceron,Carlos Roberto; Carareto,Claudia Marcia Aparecida. Zaprionus indianus is a dipteran (Drosophilidae) with a wide distribution throughout the tropics and temperate Palearctic and Nearctic regions. There have been proposals to reclassify the genus Zaprionus as a subgenus or group of the genus Drosophila because various molecular markers have indicated a close relationship between Zaprionus species and the immigrans-Hirtodrosophila radiation within Drosophila. These markers, together with alloenzymes and quantitative traits, have been used to describe the probable scenario for the expansion of Zaprionus indianus from its center of dispersal (Africa) to regions of Asia (ancient dispersal) and the Americas (recent dispersal). The introduction of Z. indianus into Brazil was first reported in 1999 and the current... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alloenzyme; Bioinvasion; Molecular markers; Phylogenetic analysis; Quantitative traits. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300003 Esterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae) Genet. Mol. Biol. Baffi,Milla Alves; Pereira,Cícero Donizete; Souza,Guilherme Rocha Lino de; Bonetti,Ana Maria; Ceron,Carlos Roberto; Gourlart,Luiz Ricardo. The cattle tick Boophilus microplus causes great damage in livestock and is considered one of the most important tropical ectoparasites. The traditional method of control is based on the intensive use of pesticides, however the indiscriminate use of these compounds over the years has led to the selection of resistant ticks. Hydrolases, particularly esterases (EST), have been reported to be associated with acaricide resistance in B. microplus. We compared the esterase profile of susceptible and cypermethrin-resistant strains of adult B. microplus and a pyrethroid susceptible reference strain (the Mozzo strain) using polyacrylamide gel electrophoresis and specific staining. The electrophoretic profiles of protein extracts revealed the presence of four... Tipo: Info:eu-repo/semantics/other Palavras-chave: Boophilus microplus; Pesticides; Resistance; Esterases. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500016 Distribution of the Bari-I transposable element in stable hybrid strains between Drosophila melanogaster and Drosophila simulans and in Brazilian populations of these species Genet. Mol. Biol. Lucca Jr.,Marcos de; Carareto,Claudia Marcia Aparecida; Ceron,Carlos Roberto. We analyzed the distribution of the Bari-I transposable element in Drosophila melanogaster (IN(1)AB), its sibling species Drosophila simulans (C167.4) and in eight hybrid strains derived from initial crosses involving D. simulans females and D. melanogaster males of the above cited strains as well as in Brazilian populations of these species. Polymerase chain reaction (PCR) data showed the presence of the Bari-I element among species populations and hybrid strains. Hybridization with a 703 bp probe homologous to the Bari-I sequence showed that the number of Bari-I copies in D. melanogaster IN(1)AB was higher than in D. simulans C167.4 strains. Hybrid strains presented Bari-I sequences related to both parental species. In addition some strains displayed a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bari-I; Drosophila melanogaster; D. simulans; Introgressive hybridization; Transposable element. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400028 Differential transcription of ribosomal cistrons denoting nucleolar dominance in hybrids of Drosophila mulleri and Drosophila navojoa (mulleri complex, Repleta group) Genet. Mol. Biol. Commar,Leliane Silva; Bicudo,Hermione E.M.C.; Rahal,Paula; Ceron,Carlos Roberto. The fruit flies Drosophila mulleri and Drosophila navojoa are included in the mulleri complex of the mulleri subgroup and Repleta group. Although there is no demonstration that interspecific crosses between them occur in nature, they intercross in the laboratory in both cross directions. Previous data have shown the occurrence of nucleolar dominance in interspecific hybrids of some species in the mulleri complex. We investigated nucleolar dominance in D. mulleri/D. navojoa hybrids using the transcription profiles of the rDNA internal transcribed spacer (ITS-1) region. The results showed that the ribosomal cistrons present in the X chromosome and in the microchromosome of D. navojoa are exclusively or preferentially transcribed in these hybrids depending on... Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila mulleri; D. navojoa; Interspecific hybrids; Nucleolar dominance; RDNA. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600028 Esterase profile in the postembryonic development of Rhipicephalusmicroplus PAB Baffi,Milla Alves; Pereira,Cícero Donizete; Souza,Guilherme Rocha Lino de; Ceron,Carlos Roberto; Bonetti,Ana Maria. The objective of this work was to analyze the pattern of esterase activity in the development stages of Rhipicephalus microplus by nondenaturing polyacrylamide gel electrophoresis using specific staining for esterase. The electrophoretical results revealed the presence of nine regions displaying esterase activity, stained with both alpha-naphthyl acetate and beta-naphthyl acetate, and classified as alpha-beta-esterase. Stage-specific esterases were found, with the first nymphal and larval stages showing the greatest esterase activity throughout the development. An esterase called EST-4 was detected only in males and was considered sex-specific. There are differences in the esterase profile among the different postembryonic development stages of R. microplus. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle tick; Resistance to acaricides; Tick control. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000800016 Molecular weight estimation of esterase isoenzymes in closely related Drosophila Species (Diptera: Drosophilidae) in non-denaturing polyacrylamide gel electrophoresis BABT Mateus,Rogério Pincela; Cabral,Hamilton; Bonilla-Rodriguez,Gustavo Orlando; Ceron,Carlos Roberto. A method that allows the measure of molecular weight of two well-known and closely related esterases from Drosophila mojavensis and its sibling species, D. arizonae, is here described, using native polyacrylamide gel electrophoresis at several concentrations, applying Fergunson´s principles. These enzymes, namely EST-4 and EST-5, presented molecular weight values between 81 and 91 kDa. In spite of their distinct expression pattern through the insect's life cycle, they showed properties of isoenzymes codified by distinct structural genes, supporting the hypothesis of a rather recent gene duplication event that generated both in D. mojavensis and D. arizonae, as well as in other species of repleta group. The method is simple and adequate to be applied to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterases; Isoenzymes; Molecular weight determination; Drosophila mojavensis; Drosophila arizonae; PAGE. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500004 Registros recuperados: 7 Primeira ... 1 ... Última

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