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| Chae,Song Wha; Jee,Bo Keun; Lee,Joo Yong; Han,Chang Whan; Jeon,Yang-Whan; Lim,Young; Lee,Kweon-Haeng; Rha,Hyoung Kyun; Chae,Gue-Tae. |
| Human bone marrow-derived mesenchymal stem cells (hMSCs) have the capacity to differentiate into osteoblasts during osteogenesis. Several studies attempted to identify osteogenesis-related genes in hMSCs. Although HOX genes are known to play a pivotal role in skeletogenesis, their function in the osteogenesis of hMSCs has not yet been investigated in detail. Our aim was to characterize the expression of 37 HOX genes by multiplex RT-PCR to identify the ones most probably involved in osteogenic differentiation. The results showed that the expression patterns of four HOX genes were altered during this process. In particular, the expression levels of HOXC13 and HOXD13 were dramatically changed. Real-time PCR and Western blot analysis were performed... |
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Palavras-chave: Human mesenchymal stem cells; HOX genes; Osteogenic differentiation; Stem cell differentiation; Gene profiling. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500002 |
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