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	Provedor de dados Electron. J. Biotechnol. (1) Autor Chan,Pek-Lan (1) Cheah,Suan-Choo (1) Low,Eng-Ti L (1) Ma,Lay-Sun (1) Ooi,Leslie Cheng-Li (1) Shariff,Elyana M (1) Singh,Rajinder (1) Mais... Palavra-chave Auxin (1) Expressed sequence tags (1) Gene expression (1) Low abundance genes (1) Tissue culture (1) Tipo do documento Journal article (1) Ano 2010 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Normalized embryoid cDNA library of oil palm (Elaeis guineensis) Electron. J. Biotechnol. Chan,Pek-Lan; Ma,Lay-Sun; Low,Eng-Ti L; Shariff,Elyana M; Ooi,Leslie Cheng-Li; Cheah,Suan-Choo; Singh,Rajinder. A normalized embryoid cDNA library (EON) was constructed based on reassociation kinetics reaction. Results from dot blot hybridization and sequencing of EON cDNA clones clearly indicated that the normalization process reduced the frequency of high abundance transcripts and increased the frequency of low abundance gene transcripts. A total of 553 non-redundant expressed sequence tags (ESTs) were identified, 325 of these were not observed in the standard oil palm cDNA libraries sequenced previously. A total of 10 EON cDNA clones were chosen for expression profiling across samples from different stages of the tissue culture process. Two of the genes exhibited promising expression patterns for predicting the embryogenic potential in callus. Some of these genes... Tipo: Journal article Palavras-chave: Auxin; Expressed sequence tags; Gene expression; Low abundance genes; Tissue culture. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100010 Registros recuperados: 1 Primeira ... 1 ... Última

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