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| Nouaille,S.; Bermúdez-Humarán,L.G.; Adel-Patient,K.; Commissaire,J.; Gruss,A.; Wal,J.M.; Azevedo,V.; Langella,P.; Chatel,J.M.. |
| The stabilizing effects of staphylococcal nuclease (Nuc) and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS) on the production of a model food allergen, bovine ß-lactoglobulin (BLG), in Lactococcus lactis were investigated. The fusion of Nuc to BLG (Nuc-BLG) results in higher production and secretion of the hybrid protein. When LEISS was fused to BLG, the production of the resulting protein LEISS-BLG was only slightly improved compared to the one obtained with Nuc-BLG. However, the secretion of LEISS-BLG was dramatically enhanced (~10- and 4-fold higher than BLG and Nuc-BLG, respectively). Finally, the fusion of LEISS to Nuc-BLG resulting in the protein LEISS-Nuc-BLG led to the highest production of the hybrid protein, estimated at ~8 µg/ml... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactococcus lactis; Protein secretion; LEISSTCDA; Staphylococcal nuclease; SS-lactoglobulin; Allergen. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300005 |
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