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	Provedor de dados BJMBR (5) Autor Chauffaille,M.L.L.F. (5) Pinheiro,R.F. (3) Kerbauy,J. (2) Yamamoto,M. (2) Antunes,S.V. (1) Beltrani,R. (1) Briones,M.R.S. (1) Campos,M.G.V. (1) Figueiredo,M.S. (1) Ghaname,F.S. (1) Kerbauy,D.M.B. (1) Pelloso,L.A.F. (1) Rodrigues,C.A. (1) Serio,F.M. (1) Silva,M.R.R. (1) Stefano,J.T. (1) Mais... Palavra-chave Acute myeloid leukemia (2) FISH (2) Karyotype (2) Myelodysplastic syndrome (2) Acute promyelocytic leukemia (1) Bone marrow cells (1) Cryopreservation (1) Cytogenetic abnormalities (1) Elderly patients (1) G-banding (1) G-banding karyotype (1) Induction therapy (1) Loss of heterozygosity (1) Overall survival (1) PML/RARA genem rearrangement (1) RT-PCR (1) Mais... Tipo do documento Info:eu-repo/semantics/other (3) Info:eu-repo/semantics/article (2) Ano 2009 (1) 2008 (1) 2003 (2) 2001 (1) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Karyotype of cryopreserved bone marrow cells BJMBR Chauffaille,M.L.L.F.; Pinheiro,R.F.; Stefano,J.T.; Kerbauy,J.. The analysis of chromosomal abnormalities is important for the study of hematological neoplastic disorders since it facilitates classification of the disease. The ability to perform chromosome analysis of cryopreserved malignant marrow or peripheral blast cells is important for retrospective studies. In the present study, we compared the karyotype of fresh bone marrow cells (20 metaphases) to that of cells stored with a simplified cryopreservation method, evaluated the effect of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) as an in vitro mitotic index stimulator, and compared the cell viability and chromosome morphology of fresh and cryopreserved cells whenever possible (sufficient metaphases for analysis). Twenty-five bone marrow... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cryopreservation; Karyotype; Bone marrow cells. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700004 Comparison of I-FISH and G-banding for the detection of chromosomal abnormalities during the evolution of myelodysplastic syndrome BJMBR Pinheiro,R.F.; Chauffaille,M.L.L.F.. Myelodysplastic syndrome (MDS) patients with a normal karyotype constitute a heterogeneous group from a biological standpoint and their outcome is often unpredictable. Interphase fluorescence in situ hybridization (I-FISH) studies could increase the rate of detection of abnormalities, but previous reports in the literature have been contradictory. We performed I-FISH and conventional karyotyping (G-banding) on 50 MDS patients at diagnosis, after 6 and 12 months or at any time if a transformation to acute myeloid leukemia (AML) was detected. Applying a probe-panel targeting the centromere of chromosomes 7 and 8, 5q31, 5p15.2 and 7q31, we observed one case with 5q deletion not identified by G-banding. I-FISH at 6 and 12 months confirmed the karyotype... Tipo: Info:eu-repo/semantics/other Palavras-chave: Myelodysplastic syndrome; FISH; G-banding. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100018 Association of loss of heterozygosity with cytogenetic abnormalities in acute myeloid leukemia and myelodysplastic syndrome BJMBR Pinheiro,R.F.; Serio,F.M.; Silva,M.R.R.; Briones,M.R.S.; Chauffaille,M.L.L.F.. Deletions on chromosomes 5 and 7 are frequently seen in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). It is assumed that these deletions indicate loss of tumor suppressor genes on these chromosomes and until these tumor suppressor genes are identified, the functional consequences of these deletions and the molecular basis of these myeloid disorders cannot be completely understood. We evaluated loss of heterozygosity (LOH) in 44 patients (18 MDS and 26 AML, diagnosed according to WHO classification criteria) at diagnosis, using a four-microsatellite marker panel: an intragenic marker on the 7th intron of gene IRF-1 of the 5q31.1 region and three markers located inside the 7q31.1 region and correlated the LOH with karyotype abnormalities.... Tipo: Info:eu-repo/semantics/other Palavras-chave: Myelodysplastic syndrome; Acute myeloid leukemia; Cytogenetic abnormalities; Loss of heterozygosity. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700010 Acute myeloid leukemia in elderly patients: experience of a single center BJMBR Rodrigues,C.A.; Chauffaille,M.L.L.F.; Pelloso,L.A.F.; Ghaname,F.S.; Kerbauy,D.M.B.; Campos,M.G.V.; Yamamoto,M.. Acute myeloid leukemia (AML) is a disease predominantly of older adults. Treatment of AML in the elderly is complicated not only by comorbidities but also by the high prevalence of poor prognosis markers. Thirty-one consecutive unselected patients with AML older than 60 years (representing 33% of all AML cases diagnosed at our institution during the same period) were followed over a period of 5 years (1997-2002). A high incidence of AML with multilineage dysplasia (45%) and no favorable cytogenetic abnormalities but 62% intermediate and 38% unfavorable karyotypes were found. Sixteen patients (52%) were selected for induction of intensive cytotoxic treatment and complete remission was achieved only by some of these intensively treated patients (7 of 16). Of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute myeloid leukemia; Elderly patients; G-banding karyotype; Induction therapy; Overall survival. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600004 Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques BJMBR Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J.. Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean: 56%). RT-PCR was done... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006 Registros recuperados: 5 Primeira ... 1 ... Última

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