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| Nóbrega Jr.,J.E.; Rossetto,R.; Matos,M.H.T.; Chaves,R.N.; Magalhães,D.M.; Lima-Verde,I.B.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.; Gonçalves,P.B.D.; Oliveira,J.F.C.. |
| This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro. Ovarian fragments were cultured for 1 or 7 days in Minimum Essential Medium with different S1P concentrations (0, 1, 10, 50, 100 or 200ng/mL). All ovarian fragments were processed for histological analysis in optical microscopy, transmission electron microscopy and fluorescence analysis. The treatment using 1ng/mL of S1P was able to maintain the percentage of normal follicles with the progression of the culture from day 1 to 7. At end of the 7-day culture period there was a significant reduction (P<0.05) in the percentage of primordial follicles in all groups... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preservation; Preantral follicles; Sphingosine 1-phosphate; Follicular activation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000400977 |
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| Rodrigues,G.Q.; Lima,I.M.T.; Chaves,R.N.; Rossetto,R.; Costa,S.L.; Castro,S.V.; Barros,V.R.P.; Matos,M.H.T.; Lopes,C.A.P.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.. |
| The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat; Preantral follicle; In vitro development; TGF-β. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200013 |
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| Martins,F.S.; Celestino,J.J.H.; Saraiva,M.V.A.; Chaves,R.N.; Rossetto,R.; Silva,C.M.G.; Lima-Verde,I.B.; Lopes,C.A.P.; Campello,C.C.; Figueiredo,J.R.. |
| The objective of this study was to determine the effects of GDF-9, IGF-I, and GH alone or combined on preantral follicle survival, activation and development after 1 and 7 days of in vitro culture. Either fresh (non-cultured) or cultured ovarian tissue was processed for histological and fluorescence analysis. For all media tested, the percent of normal follicles was greater when compared to minimum essential medium supplemented (MEM+) alone, except when ovarian tissue was cultured with GDF-9/IGF-I or GDF-9/GH (P < 0.05). Fluorescence analysis showed that the percent of viable follicles after 7 days of culture was similar for non-cultured tissue and for all treatments tested. The percent of primordial follicles was reduced (P < 0.05) and there was a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GDF-9; IGF-I; GH; Culture; Follicle activation; Goat. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800006 |
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