|
|
|
|
|
Shen,Bo; Zhu,Qun; Zheng,Ma-Qing; Chen,Jia; Shi,Mei-Qi; Feng,Ji-Feng. |
Polymorphisms in the nicotinic acetylcholine receptor subunit CHRNA5 gene have been associated with lung cancer positive susceptibility in European and American populations. In the present hospital-based, case-control study, we determined whether polymorphism in rs503464 of CHRNA5 is associated with lung cancer risk in Chinese individuals. A single nucleotide polymorphism in CHRNA5 rs503464, c.-166T>A (hereafter T>A), was identified using TaqMan-MGB probes with sequencing via PCR in 600 lung cancer cases and 600 healthy individuals. Genotype frequencies for rs503464 (T>A) were in Hardy-Weinberg equilibrium for the control population. However, genotype frequencies were significantly different between cases and controls (P < 0.05), while allele... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lung cancer; Nicotinic acetylcholine receptors; CHRNA5 gene; Single nucleotide polymorphisms. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100079 |
| |
|
|
Wang,Jiashun; Li,Yi; Chen,Jia; Hua,Deping; Li,Yi; Deng,Hui; Li,Ying; Liang,Zhixuan; Huang,Jinhai. |
Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The results showed that the PagC polyclonal antiserum is of good specificity and the capture rate of 0.1 mg IMBs for Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The method developed demonstrated high specificity for the positive Salmonella samples when compared to non-specific DNA samples, such as Escherichia coli, Staphylococcus aureus,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; IMBs-qPCR; PagC gene; Detection. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200320 |
| |
|
|
|