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| Wang,Jianmin; Song,Ruihua; Chen,Lei; Yin,Liangjun; Chen,Bo; Sun,Jing; Gou,Yuanbing; Zhao,Ling; Chen,Lin. |
| Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot and PCR. Results: The targeting vector with conditional knockout of murine FGFR2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibroblast growth factor receptor-2 (FGFR2); Conditional knockout; Homologous recombination; Cre; ES cells. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200002 |
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| Li,Kailong; Du,Xiaolan; He,Yani; Zhao,Lin; Yang,Jvrong; Song,Ruihua; Chen,Lin. |
| Objective: To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence. Methods: Experimental models of unilateral renal IRI were used in p53(+/+) and p53(-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI. Results: Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53(+/+) mice in later stages than in earlier stages. Senescent tubular cells were significantly increased at 3 and 6 months after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell senescence; Cell cycle regulatory protein; Ischemia/reperfusion injury; Kidney. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200003 |
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| Chen,Lin; Huang,Zhuochun; Liao,Yun; Yang,Bin; Zhang,Junlong. |
| Tumor necrosis factor-alpha (TNF-α) plays an important role in autoimmune diseases. Previous studies have investigated the association of TNF-α-238G/A (rs361525) and -308G/A (rs1800629) polymorphisms with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). However, no agreed conclusion had been made. Therefore, this meta-analysis was conducted to assess the associations of TNF-α-238G/A and -308G/A polymorphisms with RA and SLE risk. A systematic search was conducted in commonly used databases. Meta-analysis was performed by STATA12.0. A total of 43 studies were included. In the overall population, the TNF-α-238A allele was observed to be a protective factor for RA (A vs G: OR=0.75, 95%CI=0.57–0.99, P=0.040) and the TNF-α-308A allele was found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rheumatoid arthritis; Single-nucleotide polymorphism; Systemic lupus erythematosus; Tumor necrosis factor-alpha; Meta-analysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300607 |
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| Hao,Yi; Zhang,Long; Zhang,Zhenhua; Chen,Lin; He,Ning; Zhu,Shuai. |
| Exercise-based training decreases hospitalizations in heart failure patients but such patients have exercise intolerance. The objectives of the study were to evaluate the effect of 12 weeks of Tai Chi exercise and lower limb muscles' functional electrical stimulation in older chronic heart failure adults. A total of 1,084 older adults with chronic systolic heart failure were included in a non-randomized clinical trial (n=271 per group). The control group did not receive any kind of intervention, one group received functional electrical stimulation of lower limb muscles (FES group), another group practiced Tai Chi exercise (TCE group), and another received functional electrical stimulation of lower limb muscles and practiced Tai Chi exercise (FES & TCE... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Geriatric; Heart failure; Kansas City Cardiomyopathy Questionnaire; Functional electrical stimulation of lower limb muscles; Tai Chi exercise. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001200604 |
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