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| Tu,Yi-Chen; Wei,Liang-Yuan; Chang,Yi-Ying; Liu,Hsiu-Chou; Lee,Hsien-Hsiung; Yu,Yu-Hsiang; Chen,Ming-Cheng. |
| ABSTRACT The objective of the present study was to investigate the effect of single nucleotide polymorphism (SNP) of the melanocortin 1 receptor (MC1R) gene on plumage coloration in mule ducks. PCR-high-resolution melting analysis (PCR-HRM) and DNA sequencing were used to identify the SNP variability of the MC1R gene in white common ducks. Three non-synonymous SNP (MC1R gene exon 1, c.52G>A, c.376G>A, and c.409G>A) were identified in white Tsaiya ducks. Mating test (white Tsaiya ducks × white Muscovy drakes) in combination with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to investigate the effect of non-synonymous SNP of different maternal lines on plumage coloration in mule ducks. Genotyping results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscovy drakes; Polymerase chain reaction-restriction fragment length polymorphism; Single nucleotide polymorphism; Tsaiya ducks. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100305 |
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