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	Provedor de dados BABT (1) BJMBR (1) Genet. Mol. Biol. (1) Autor Chen,Xiao (3) Chen,Yingying (1) Gao,Ying-fang (1) Huang,Qiong (1) Li,Jian (1) Li,Rui (1) Li,Yanwei (1) Song,Xinghui (1) Wang,Linlin (1) Xu,Guowei (1) Yang,Qian (1) Yin,Guoli (1) Zhang,Guo-hua (1) Zhang,Xue-ling (1) Zhao,Qi (1) Mais... Palavra-chave 3-Hydroxy-3-methylglutaryl-CoA reductases (1) Adipocyte differentiation (1) Artesunate (1) CD4+ T cells (1) Embryonic stem cells (1) Expression pattern (1) Fibroblast growth factor (1) Fritillaria cirrhosa (1) Mesenchymal stem cells (1) MiR-142 (1) Molecular cloning (1) Ovarian cancer (1) Prokaryotic expression (1) Sirt1 (1) Th1 cells (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2019 (1) 2018 (1) 2014 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última bFGF promotes adipocyte differentiation in human mesenchymal stem cells derived from embryonic stem cells Genet. Mol. Biol. Song,Xinghui; Li,Yanwei; Chen,Xiao; Yin,Guoli; Huang,Qiong; Chen,Yingying; Xu,Guowei; Wang,Linlin. In this work we describe the establishment of mesenchymal stem cells (MSCs) derived from embryonic stem cells (ESCs) and the role of bFGF in adipocyte differentiation. The totipotency of ESCs and MSCs was assessed by immunofluorescence staining and RT-PCR of totipotency factors. MSCs were successfully used to induce osteoblasts, chondrocytes and adipocytes. MSCs that differentiated into adipocytes were stimulated with and without bFGF. The OD/DNA (optical density/content of total DNA) and expression levels of the specific adipocyte genes PPARγ2 (peroxisome proliferator activated receptor γ2) and C/EBPs were higher in bFGF cells. Embryonic bodies had a higher adipocyte level compared with cells cultured in plates. These findings indicate that bFGF promotes... Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryonic stem cells; Mesenchymal stem cells; Fibroblast growth factor; Adipocyte differentiation. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100019 Cloning and Characterization of the Gene Encoding 3-hydroxy-3- Methylglutaryl-coenzyme A (HMG-CoA) Reductase from Fritillaria Cirrhosa D. Don BABT Zhao,Qi; Li,Rui; Chen,Xiao; Yang,Qian; Li,Jian. ABSTRACT The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in Mevalonate (MVA) pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR from Fritillaria cirrhosa (FcHMGR), a bulbous medicinal plant. The full-length cDNA of FcHMGR was 2072 base pair (bp), containing a 1680-bp open reading frame. Bioinformatical analyses revealed that FcHMGR had HMG CoA-binding domains and two NADPH binding domains, which are required for HMGR activity. Quantitative real-time PCR (qRT-PCR) analysis revealed that FcHMGR expressed high in mature bulbs. A truncated version of FcHMGR protein lacking the N-terminal 249-bp GC rich area was... Tipo: Info:eu-repo/semantics/article Palavras-chave: 3-Hydroxy-3-methylglutaryl-CoA reductases; Fritillaria cirrhosa; Molecular cloning; Expression pattern; Prokaryotic expression. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100438 Artesunate promotes Th1 differentiation from CD4+ T cells to enhance cell apoptosis in ovarian cancer via miR-142 BJMBR Chen,Xiao; Zhang,Xue-ling; Zhang,Guo-hua; Gao,Ying-fang. The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4+ T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4+ T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4+ T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-γ level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4+ T cells and Th1 differentiation from CD4+ T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing... Tipo: Info:eu-repo/semantics/article Palavras-chave: Artesunate; Ovarian cancer; CD4+ T cells; Th1 cells; MiR-142; Sirt1. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500606 Registros recuperados: 3 Primeira ... 1 ... Última

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