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Dai,Dawei; Wang,Junyu; Jiang,Ying; Yuan,Lei; Lu,Youming; Zhang,Aijun; Zou,Dongdong; Chen,Xin. |
The present study aimed to identify microRNAs (miRNAs) that are involved in neuropathic pain and predict their corresponding roles in the pathogenesis and development process of neuropathic pain. The rat model of neuropathic pain caused by spared nerve injury (SNI) was established in Sprague-Dawley male rats, followed by small RNA sequencing of the L3–L6 dorsal root ganglion. Real-time PCR was performed to validate the differently expressed miRNAs. Functional verification was performed by intrathecally injecting the animals with miRNA agomir. A total of 72 differentially expressed miRNAs were identified in the SNI rats, including 33 upregulated and 39 downregulated miRNAs. The results of qPCR further verified the expression levels of rno-miR-6215... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MicroRNAs; Neuropathic pain; Spared nerve injury; Dorsal root ganglion. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000602 |
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Lu,Caiyan; Ma,Jian; Chen,Xin; Zhang,Xudong; Shi,Yi; Huang,Bin. |
A pot experiment was conducted to evaluate the effect of nitrogen (N) fertilizer and maize straw incorporation on the accumulation of NH4+-15N and N0(3)--15N in soil inorganic N pool among three consecutive cropping cycles, aimed to search for an effective N management practice to decrease superfluous accumulation of soil inorganic N and fertilizer N losses. The results showed that the amounts of soil NH4+-15N, N0(3)-15N and inorganic 15N, and their percent to applied 15N-labeled fertilizer declined significantly with sampling time (p ≤ 0.001).Compared to low N application rate (44.64 mg N kg-1 soil), high N application rate (89.28 mg N kg-1 soil) enhanced significantly the amounts of soil NH4+-15N, N0(3)--15N and inorganic 15N by 238.6%, 132.9%... |
Tipo: Journal article |
Palavras-chave: N fertilizer; Maize straw; NH4+-15N; N0(3)--15N accumulation; Black soil. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000200005 |
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